[Histonet] CD10

[Richard Cartun]

A 1:5 (or 1:10) dilution of an antibody doesn't sound very practical in terms of cost per slide.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> Rene J Buesa <rjbuesa <@t> yahoo.com> 03/29/06 03:39PM >>>
Theresa:
  I used CD10 from Novocastra at a dilution of 1:5 with pH6 HIER
  I know of a lab in the UK that dilutes it 1:10 (and seem to work OK, I have seen their slides).
  Hope this will help!
  René J.

Theresa Rohr <RohrT <@t> nyackhospital.org> wrote:
  Hello,

I was wondering if anyone has any suggestions regarding CD10. I have the Dako Autostainer and use the Envision+ system and a Steamer for Antigen Retrieval.

Since Dako only has an antibody for use on Frozen sections, I am searching for one for FFPE tissue. My Dako Rep actually suggested Novocastro as having a great antibody. 

Does anyone out there agree/disagree??? 
At what dilution???? 

What I already researched looks like this is not a cheap antibody in terms of dillution. 

I would appreciate any help.

Thanks so much,
Theresa Rohr, Nyack Hospital
rohrt <@t> nyackhospital.org 

Theresa Rohr, BA, HT(ASCP)
Section Head, Histology
Nyack Hospital
160 North Midland Avenue
Nyack, New York 10960
phone 845-348-2276
fax 845-348-8430

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