[Histonet] RE: mouse antibodies on mouse tissue
C.M. van der Loos
c.m.vanderloos <@t> amc.uva.nl
Wed Mar 29 08:45:22 CST 2006
Dear Claudia,
The mouse monoclonal antibodies were never raised against mouse
antigens at all. The point is that the original mice were immunized
with antigens isolated mostly from human sources. Later it appeared
that the antigens in question are very similar among several animal
species. In fact so similar that the mouse monoclonal antibody also
reacts with that same epitope in mouse tissue.
Hope this helps,
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands
Date: Wed, 29 Mar 2006 07:42:46 +1000
From: Claudia Freyer <Claudia.Freyer <@t> princehenrys.org>
Subject: [Histonet] mouse antibodies on mouse tissue
To: histonet <@t> lists.utsouthwestern.edu
Dear Histonetter,
I am confused about the use of monoclonal antibodies produced in mice
against endogenous proteins in mouse tissue. I thought an animal would
not produce antibodies against its own proteins, since this would
cause an autoimmune response. Would anybody know why mouse monoclonals
detect epitopes on mouse proteins?
Many thanks.
Claudia
Dr Claudia Freyer
Prince Henry's Institute of Medical Research
Victoria
Australia
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