[Histonet] RE: Mouse antibodies on mouse tissue

[Johnson, Teri]

Dear Claudia,

I think your confusion might be due to the fact the monoclonal antibodies produced in mice are generally against HUMAN proteins. Using them on mouse tissue relies on interspecies protein homology, i.e. the amino acid structure is similiar enough to allow binding. Most anti-mouse antibodies (created by injecting mouse proteins) are made in rat.

Does this help?

Teri Johnson
Stowers Institute for Medical Research
Kansas City, MO 64110