[Histonet] BSA in PBS

[louise renton]

Hello all,

would someone please remind me of the concentration of Bovine serum
albumin to put in PBS buffer to reduce background staining (IHC)?

Also, if I don't have a microwave, pressure cooker, rice/vegetable
steamer, or autoclave - whats another  way to do heat mediated antigen
retrieval?

Best regards and thanks in advance

--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"Does a conceited cowboy, only ride on pompous grass?.