[Histonet] Insulin Growth Factor-1 help

Dana Settembre settembr <@t> umdnj.edu
Tue Mar 28 10:36:50 CST 2006

I have been trying to get IGF-1 to work on formalin fixed paraffin
embedded human tissue and it's not working.
I am using Santa Cruz' IGF-1  rabbit polyclonal, (cat# sc-9013)
I have tried all sorts of retrievals and all sorts of dilutions with my
anti mouse/anti rabbit universal detection kit.
Things usually work one way or another and I am at my wits end.
Can someone who has gotten this antibody to work on FFPE human tissue
please let me know
what Vendor you use, retrieval, dilution, detection system?  
Thank you so much for any help.

Dana Settembre
UMDNJ - University Hospital
Newark, NJ

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