[Histonet] CD11b

[Joanne Mauger]

Patsy,
I'm staining human tissue. Where do you get the F4/80?

Thanks,
Jo

>>> "patsy ruegg" <pruegg <@t> ihctech.net> 03/26/06 7:52 PM >>>
Jo,
I tried several cd11b's on ffpe tissue to no avail, I switched to
F4/80
clone for macs and it does work on ffpe.  The one I have is rat anti ms
I
believe, what species are you trying to label?
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net 
www.ihctech.net 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joanne
Mauger
Sent: Friday, March 24, 2006 9:12 AM
To: mariaforzan <@t> hotmail.com; histonet <@t> pathology.swmed.edu;
ploykasek <@t> phenopath.com 
Subject: [Histonet] CD11b

Hi All,

Does anyone know of a CD11b antibody that works on FFPE?

Thanks,
Jo

>>> Patti Loykasek <ploykasek <@t> phenopath.com> 03/24/06 11:07 AM >>>
Maria, 
We are using a different clone - clone G3 from Chemicon. In our
validation
we did see some cross reactivity with basophils. It was positive on
all
cases of mast cell disease we tested & negative on other neoplasms &
hematopoietic cell types.


Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA

 
>  Hello!
> 
>  We  are  having some trouble with our IHC stain for mast cell
tryptase
>  (Dako  AA1  clone).  We seem to get granular intracytoplasmic
staining
>  in  several  cells that are probably not mast cells but histiocytes
or
>  neoplastic  plasma  cells.   I know that the antibody is very
specific
>  for  mast  cells and should not label anything else.  So, does
anybody
>  have any ideas?  Could it be the retrieval?  Or the secondary?  We
use
>  EnVision so it should not be a problem.
> 
>  Does anybody have any suggestions or has encounter a similar
problem?
> 
>  Maria Forzan
> 
>  Finn Pathologists
> 
>  44 (0)1379 854180
> 
>  "Pebbles are inevitable"
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


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