Fw: [Histonet] CAP Guidelines for Microwaves

Fri Mar 24 11:56:47 CST 2006

Here is my $0.02

Ideally of course it would be better and wiser to have a laboratory designed
industrial microwave oven in the histology lab. Now... does that mean that
it is practical? Not necessarily. It is my impression that CAP merely wants
laboratories to monitor and document the use and temperature reproducability
of any and all MW ovens used in the lab. Seeing as how this is new wave of
technology in the lab, I seriously doubt that anyone is suggesting that MW
ovens not be used. They are too important to the functioning in today's
laboratory. I think that CAP just wants to make sure that their use is
strictly monitored and recorded.

----- Original Message ----- 
From: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
To: "Luck, Greg D." <LuckG <@t> empirehealth.org>; "'Bartlett, Jeanine'"
<jqb7 <@t> cdc.gov>; "Angela Bitting" <akbitting <@t> geisinger.edu>;
<histonet <@t> lists.utsouthwestern.edu>; <jnocito <@t> pathreflab.com>;
<jnocito <@t> satx.rr.com>; <HParker <@t> Skaggs.Net>
Sent: Friday, March 24, 2006 10:54 AM
Subject: RE: [Histonet] CAP Guidelines for Microwaves

> Greg;
>   That is essentially my point. In the same way that you never would try
to enter the Monaco race with a small FIAT 600 (but with a Porsche) you
should never try to do things with a household MW oven that will require a
lab designed one.
>   Things I did (for years) with my 2 MW ovens: dry slides, all sorts of
boosts to special stains, heating the citrate buffer before the HIER
procedure and things of that sort.
>   Never to process tissue (although I had to confess some "indiscretionts
in that realm").
>   Regarding durability, my MW was used for more than 10 years
continuously.
>   Regarding reproducibility: it CAN be obtained as long as you calibrate
it and know their capabilities [I even published a paper on that topic].
>   Now to the original question about CAP regulations and household MW
ovens: the answre and approach from their point of view is the same: "do NOT
used them" [for that purpose].
>   René J.
>
> "Luck, Greg D." <LuckG <@t> empirehealth.org> wrote:
>   Oh Why Not,
>
> I've taken the position (that from the CAP comment section of this
question)
> that use of a non commercial grade microwave is still safe and usable
> provided you limit its use to heating of reagents such as PBS, agar, and
> tris or citrate buffers.
>
> Greg Luck
> Anatomic Pathology Supervisor
> Deaconess Med Center / EHS
> 800 W. 5th Ave
> Spokane, WA 99204
> Phone 509.473.7394
> Fax 509.473.7133
> luckg <@t> empirehealth.org
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]
> Sent: Friday, March 24, 2006 4:17 AM
> To: Angela Bitting; histonet <@t> lists.utsouthwestern.edu;
> jnocito <@t> pathreflab.com; jnocito <@t> satx.rr.com; HParker <@t> Skaggs.Net;
> rjbuesa <@t> yahoo.com
> Subject: RE: [Histonet] CAP Guidelines for Microwaves
>
> Heating Bouin's is an issue, I would think.
>
>
> Jeanine Bartlett, BS, HT(ASCP)
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Angela
> Bitting
> Sent: Friday, March 24, 2006 7:09 AM
> To: histonet <@t> lists.utsouthwestern.edu; jnocito <@t> pathreflab.com;
> jnocito <@t> satx.rr.com; HParker <@t> Skaggs.Net; rjbuesa <@t> yahoo.com
> Subject: Re: [Histonet] CAP Guidelines for Microwaves
>
> Are any of the chemicals we heat in our microwaves toxic? I don't think
most
> special stains solutions fall in that catagory. That's the first question
to
> answer before getting to wrapped up in this ventilation issue?
>
> Angela Bitting, HT(ASCP)
> Technical Specialist, Histology
> Geisinger Medical Center
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone 570-214-9634
> fax 570-271-5916
>
> >>> "Joe Nocito" 3/23/2006 7:42 PM >>>
>
> but what about using a table top fume absorber with charcoal filters? CAP
> writes these questions so ambiguously that it becomes a joke. This is the
> main reason why I have a negative attitude towards CAP.
>
> Joe
> ----- Original Message -----
> From: "Rene J Buesa"
> To: "Joe Nocito" ; "'Parker, Helayne'"
> ;
> Sent: Thursday, March 23, 2006 4:19 PM
> Subject: RE: [Histonet] CAP Guidelines for Microwaves
>
>
> > Joe:
> > It is not external ventilation to the MW oven what CAP wants, is a vent
> > connected from the microwave to an outlet and there is not a single
> > household MW oven with that characteristec so, for all practical
purposes
> > the mensage keeps being: don't use them (the household appliance) for
> > histology work, specially if tissue processing is the objective of the
> > use.
> > René J.
> >
> > Joe Nocito wrote:
> > I don't believe CAP is saying not to use them. They are saying use them
> > safely with ventilation and make sure they don't leak radiation.
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu
> > [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J
> > Buesa
> > Sent: Thursday, March 23, 2006 11:35 AM
> > To: Parker, Helayne; histonet <@t> lists.utsouthwestern.edu
> > Subject: Re: [Histonet] CAP Guidelines for Microwaves
> >
> > Helayne:
> > In short, the message from CAP is: "do not use them!"
> > Unfortunate though!
> > René J.
> >
> > "Parker, Helayne" wrote:
> > I was wondering if anyone new what CAP require of us if we have a common
> > household microwave that we use for special stains.
> >
> > Helayne Parker,HT (ASCP)
> > Histology Section Head
> > Skaggs Community Health Center
> > Branson, Missouri
> >
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