[Histonet] CD11b

[Joanne Mauger]

Hi All,

Does anyone know of a CD11b antibody that works on FFPE?

Thanks,
Jo

>>> Patti Loykasek <ploykasek <@t> phenopath.com> 03/24/06 11:07 AM >>>
Maria, 
We are using a different clone - clone G3 from Chemicon. In our
validation
we did see some cross reactivity with basophils. It was positive on
all
cases of mast cell disease we tested & negative on other neoplasms &
hematopoietic cell types.


Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA

 
>  Hello!
> 
>  We  are  having some trouble with our IHC stain for mast cell
tryptase
>  (Dako  AA1  clone).  We seem to get granular intracytoplasmic
staining
>  in  several  cells that are probably not mast cells but histiocytes
or
>  neoplastic  plasma  cells.   I know that the antibody is very
specific
>  for  mast  cells and should not label anything else.  So, does
anybody
>  have any ideas?  Could it be the retrieval?  Or the secondary?  We
use
>  EnVision so it should not be a problem.
> 
>  Does anybody have any suggestions or has encounter a similar
problem?
> 
>  Maria Forzan
> 
>  Finn Pathologists
> 
>  44 (0)1379 854180
> 
>  "Pebbles are inevitable"
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


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