[Histonet] DAB in tablet form

[Rene J Buesa]

Joyce:
  I used for years the tablets with excellent results. I prefer the tablets and to dissolve them when needed.
  René J.

meint002 <meint002 <@t> umn.edu> wrote:
  Dear Histos,

Due to the hazards of working with 3,3’ diaminobenzidine tetrahydrochloride
(DAB) for muscle enzyme staining, I am considering purchasing DAB as either
5mg, or 10mg tablets. Some companies have them dissolve in water and others
in a buffer solution. 

I am looking for input from anyone who has used these tablets. Do you like
them, do they work well, staining results, which company, etc.... I know
that you can also get DAB in sealed separate vials, but it is much more
expensive and we are in an academic research setting where cost is a
consideration. 

I am particularly interested in using them in the Cytochrome Oxidase stain. 
I have tried many procedures for this stain and my Dr. is not all that
pleased with the results. If anyone has a good procedure for this stain, I
would be grateful if you would send me a copy.

I am revamping this lab and have had a lot of questions for the histonet that
you have been very helpful in addressing. 

Thanks for all the suggestions in advance.


Joyce Meints
Histologist

University of Minnesota
Paul and Sheila Wellstone Muscular Dystrophy Center
MMC 206
420 Washington Ave. SE
Minneapolis, MN 55455-0392

e-mail: meint002 <@t> umn.edu
Fax: 612-625-8488
lab phone: 612-626-4703


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