[Histonet] DAB in tablet form
Andrea T. Hooper
anh2006 <@t> med.cornell.edu
Wed Mar 22 14:41:08 CST 2006
I too am in academic labs so relate to the cost
concern; however, I strongly recommend DAB in
liquid kit form - either DAB+ from DAKO or from
Zymed. They are liquid ready to use concentrates
diluted into buffer either supplied with the kit
or you provide yourself.
I have used both for years and years now with
excellent results. Worth the extra bucks (about
80-100 dollars for 110 mls which is actually
quite a lot) for consistent results time after
time after time - without the hazards of working
with powder or solids.
>Due to the hazards of working with 3,3í diaminobenzidine tetrahydrochloride
>(DAB) for muscle enzyme staining, I am considering purchasing DAB as either
>5mg, or 10mg tablets. Some companies have them dissolve in water and others
>in a buffer solution.
>I am looking for input from anyone who has used these tablets. Do you like
>them, do they work well, staining results, which company, etc.... I know
>that you can also get DAB in sealed separate vials, but it is much more
>expensive and we are in an academic research setting where cost is a
>I am particularly interested in using them in the Cytochrome Oxidase stain.
>I have tried many procedures for this stain and my Dr. is not all that
>pleased with the results. If anyone has a good procedure for this stain, I
>would be grateful if you would send me a copy.
>I am revamping this lab and have had a lot of questions for the histonet that
>you have been very helpful in addressing.
>Thanks for all the suggestions in advance.
>University of Minnesota
>Paul and Sheila Wellstone Muscular Dystrophy Center
>420 Washington Ave. SE
>Minneapolis, MN 55455-0392
>e-mail: meint002 <@t> umn.edu
>lab phone: 612-626-4703
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