[Histonet] Fixation sensitive IHC markers

[Sebree Linda A.]

Our pathologists have relied on a vimentin stain to assess the quality
of fixation and processing.

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Thomas
Crowell
Sent: Wednesday, March 22, 2006 1:41 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Fixation sensitive IHC markers


Dear Histonetters,

Can anyone suggest an antibody that I could use on human FFPE samples
that 
shows relative degrees of reactivity, based on variable factors such as 
fixation time or tissue autolysis?  I would like to assess the
reactivity 
of commercially available tissue bank samples.

Any suggestions would be appreciated.

Tom Crowell
Biogen Idec
Cambridge, MA
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