[Histonet] Job Openings
Kopczynski, Charlotte
Charlotte.Kopczynski <@t> baycare.org
Tue Mar 21 14:38:05 CST 2006
Baycare Health System in the Tampa Bay area has Histology Openings for the
following positions:
Mease Hospital in Dunedin, Fl. These positions will start at Mease Hospital
with transition to Morton Plant Hospital when Histology processing is
consolidated for Morton Plant Mease Health Care
Histologist Midnight - 8:30 AM
Histologist 6:30 AM - 3:00 PM
St. Joseph's Hospital in Tampa, Fl.
Lead Histologist 10:00 PM - 6:30 AM
PRN Histologist - varied hours
St. Anthony's Hospital in St. Petersburg, Fl.
Histologist - Fulltime Flexible Hours
Lead Histologist - 7:30 AM - 4:00 PM
Thanks,
Charlotte Kopczynski,HTL(ASCP)
Baycare Pathology Manager
Phone: 727-461-8246
Fax: 727-462-7597
-----Original Message-----
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Subject: Histonet Digest, Vol 28, Issue 33
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Today's Topics:
1. Re: lectin stains and antigen retrival (Gayle Callis)
2. RE: TEM and oysters (Monfils, Paul)
3. Re: mucicarmine (Johnson, Teri)
4. 2006 Missouri Symposium - June 2-3 St. Louis, MO (Johnson, Teri)
5. North Carolina Spring Mtg. (Delorise Williams)
6. RE: Re: mucicarmine (Kathy.Johnston <@t> CLS.ab.ca)
7. Human CD31, CD34, von Willebrands etc publication (Gayle Callis)
8. Rat CD8 marker attn: Patsy Ruegg (Gayle Callis)
9. (pas de sujet) (Anne-Sophie MARTINEZ)
10. MVP II (Lee & Peggy Wenk)
11. oysters larvae again (Anne-Sophie MARTINEZ)
12. Illinois Society for Histotechnologists - Annual Symposium
May 18 & 19, 2006 (Histonews)
13. RE: Cheap Accessories (Justin Thomas)
14. desperately seeking Chewie (Orr, Rebecca)
15. Looking for Richard Thrift (Charles Scouten)
16. Histotechnician Position announcement Walter Reed Army
Institute of Research- Comparative Pathology
(Atkin, Thelda J LTC WRAIR Wash-DC)
17. hello (beth zink)
18. Job posting (Patti Loykasek)
19. RE: hello (Charles.Embrey)
20. RE: oysters larvae again (Monfils, Paul)
21. Lineage Negative Cells (b003046 <@t> nf.au.dk)
----------------------------------------------------------------------
Message: 1
Date: Mon, 20 Mar 2006 11:11:18 -0700
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] lectin stains and antigen retrival
To: Franz-Josef M?ller <franzef <@t> gmx.de>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20060320105732.01b79d30 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="iso-8859-1"; format=flowed
Yes, lectins are stable although trypsin enzyme digestion at 37C may be
better than citrate buffer retrieval. Also, there are buffer
considerations for staining, i.e. TRIS Lectin buffer with Mg and Ca
added. The book gives the recipe for this and with some lectins PBS should
be avoided and also normal serums.
AND there is an excellent book on Lectin Histochemistry , a concise
practical handbook SA Brooks et al, ISBN#1859961002 that gives IHC
staining methods, etc. It is not expensive and Springer Verlag is the
publisher source.
01:18 AM 3/19/2006, you wrote:
>Dear Histonetters,
>does anybody know if lectin stains are stable after the tissue
>section (20u, paraffin embedded) was subjected to heat antigen
>retrieval in citric acid pH 6.0 ?
>Thanks for your help in advance!
>Cheers
>franzef
>
>Dr. med. Franz-Josef Müller
>Zentrum für Integrative Psychiatrie, Kiel
>Zellbiologisches Labor
>Niemannsweg 147
>24105 Kiel
>GERMANY
>
>Tel.: +49 431 597 4169
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
------------------------------
Message: 2
Date: Mon, 20 Mar 2006 13:24:09 -0500
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: RE: [Histonet] TEM and oysters
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<09C945920A6B654199F7A58A1D7D1FDE017176A2 <@t> lsexch.lsmaster.lifespan.org>
Content-Type: text/plain; charset="iso-8859-1"
(1) For most types of antigens, not likely. Glutaraldehyde crosslinks
proteins so aggressively that most antigenicity is greatly reduced or
completely destroyed. If immunolabeling is the objective before the fact,
the tissue should be fixed very conservatively, for example 0.25%
glutaraldehyde for a half hour to one hour. Following normal glutaraldehyde
fixation with osmium introduces additional problems, as osmium greatly
denatures proteins, even those crosslinked by glutaraldehyde, and actually
dissolves some pre-fixed proteins out of the tissue by breaking the
glutaraldehyde bonds. The only successful attempt at immunolabeling
glut/osmium fixed tissue that I can recall was a paper written by David
Knibbs around 1992-1994 in which he described a method for immunolabeling
(using colloidal gold-labeled antibodies) various kinds of intermediate
filaments. I believe the abstract of that paper would be in the proceedings
of the American Society of Cell Biology.
(2) You cannot "decalcify" mollusc shells in the same sense that you can
decalify bone because bone consists of a solid tissue matrix infused with
calcium salts, while a molluscan shell is composed almost completely of
calcium salts deposited by the animal's mantle, with only intermittent
traces of protein. So, if you expose the shell to decalcifying agents, the
shell will dissolve completely. If the loss of the shell is not a problem,
then it can be dissolved away much more rapidly than a bone of equivalent
size can be decalcified, since the bone matrix presents a barrier to the
penetration of the decalcifying solution. When I taught chemistry years ago,
I did a demo by filling a 1000 ml glass cylinder with 10% hydrochloric acid
and dropping in a clam shell. A dense cloud of bubbles would immediately
erupt, causing the liquid to "boil" as the calcium carbonate of the shell
was transformed into carbon dioxide, and the shell would be completely gone
in about two minutes. So, theoretically any decalcifying method could be
used to remove the shell from a molluscan specimen. If you consider acid
methods too harsh, I would recommend a chelation method such as disodium
EDTA. I never tried this on a mollusc shell, but I see no reason why it
wouldn't work. You'll have to experiment to find an optimum exposure time,
but again it should be much shorter than the time required for decalcifying
bone.
> ----------
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> Anne-Sophie MARTINEZ
> Sent: Monday, March 20, 2006 7:55 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] TEM and oysters
>
> Hi everybody. I have 2 questions concerning TEM.
> (1) I have fixed gonads of adult oysters for TEM with glutaraldehyde,
> post-fixed with osmium and done the embedding in Epon. Is there any way
> I can you do immunocytochemistry with antibodies on these samples?
> (2) How can I decalcify the shelves of larvae and juveniles oysters for
> TEM without damaging the tissue?
> Could anyone help me please? Thanks for your suggestions.
> Anne-Sophie
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 3
Date: Mon, 20 Mar 2006 12:47:51 -0600
From: "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>
Subject: [Histonet] Re: mucicarmine
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C28BAF593DC3314E9C0F3A50191C2E78044B7B36 <@t> EXCHKC03.stowers-institute.org>
Content-Type: text/plain; charset="us-ascii"
Dorothy,
It's been a while since I've done one, but I remember we got our best
results using Weigert's Iron Hematoxylin staining first, and then using
Sigma's mucicarmine stain. I don't quite remember if we used it
undiluted, but I think we did use it more concentrated than their
protocol called for. Where I trained, we made up the mucicarmine stain
and used undiluted stock to get brighter pink/rose staining results.
Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64133
------------------------------
Message: 4
Date: Mon, 20 Mar 2006 12:58:05 -0600
From: "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>
Subject: [Histonet] 2006 Missouri Symposium - June 2-3 St. Louis, MO
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C28BAF593DC3314E9C0F3A50191C2E78044B7B38 <@t> EXCHKC03.stowers-institute.org>
Content-Type: text/plain; charset="us-ascii"
If anyone is interested in coming to our fantastic meeting and you need
the registration forms and other information, please contact:
Sharon Walsh (MO Society President): userwalsh <@t> aol.com
Teri Johnson (Exhibit coordinator): tjj <@t> stowers-institute.org
Rosetta Barkley (Registrar): rbarkley2 <@t> kumc.edu
Our deepest gratitude is extended to all the exhibitors, sponsors and
speakers that make the MSH meeting possible.
MISSOURI SOCIETY
FOR
HISTOTECHNOLOGY
Presents
Meet me in St. Louis
The 29th Annual Spring Symposium
For
Continuing Education and Histopathologic Technique
June 2-3, 2006
Embassy Suites Hotel-St. Louis Downtown
GENERAL INFORMATION
Date:
June 2-3, 2006
Meeting Location:
Embassy Suites Hotel-St. Louis Downtown
901 North 1st Street
St. Louis, Missouri 63102
314-241-4200
www.stlouisdowntown.embassysuites.com
Room Reservations should be made directly with the Embassy Suites, St.
Louis, MO. Hotel reservations deadline is May 2, 2006. To secure the
symposium rates, make your reservations early. The Chicago Cubs and the
Cardinal's are playing this weekend in St. Louis, no rooms will be held
after May 2nd, and it will be almost impossible to get a room in St.
Louis. Make your reservations early.
Room Rates: $119.00 Thursday (6/1) and Friday (6/2)
Each suite is beautifully decorated with a private bedroom and spacious
living room. All of our suites are fully equipped with two televisions,
a refrigerator, microwave oven, coffee maker, two telephones with data
ports and a well lit dining/work table.
The lobby atrium provides guests a complimentary full cooked-to-order
breakfast and an Evening Managers Reception including your favorite
beverages every evening. (Free food and Drink) Attractions include the
President Casino and the St.Louis Arch.
SCHEDULE
Friday, June 2, 2006
7:30 - 8:00 REGISTRATION
8:15 - 8:30 President Message- Sharon Ann Walsh
8:30 - Noon SCIENTIFIC SESSIONS
8:30 - 9:15 Reagent Alcohol, What's it all about?
Pamela Marcum, University of PA, School
of Veterinary Medicine, Kennett Square, PA (Sponsored by Poly
Scientific)
9:15 - 10:00 Importance of Special Stains in Renal Pathology
Cherese Cortese, M.D., Associate Professor, Department of
Pathology
St. Louis University, St. Louis, Missouri
10:00 - 11:00 COFFEE BREAK & EXHIBITS
11:00 - 11:45 EM for the Histotechnologist
Randy D. Tindall, EM Specialist,
Electron Microscopy Core Facility, University of Missouri, Columbia
11:45 - 1:00 AWARDS LUNCHEON: Region V Update, Konnie Zeitner
WORKSHOPS 1:30 - 4:30
3:00 - 3:30 COFFEE BREAK & EXHIBITS
Workshop #1 Double Immuno Staining Wet Workshop, Tara Kennedy,
Applications Specialist, Biocare Medical
Workshop #2 Histology Nuts and Bolts. Back to the Basics, Mari Ann
Mailhiot BA HT(ASCP), Specimen Applications Specialist, Leica
Microsystems
Workshop #3 Stars of the Silver Screen, Trouble shooting Silver
Stains, Joan M. Vesey HT(ASCP), Richard-Allan Scientific
5:30-7:30 P.M. Upper Garden Atrium, Embassy Suites Pizza Party
Saturday, June 3, 2006
7:30 - 8:00 REGISTRATION
8:00 - 8:15 Saturday Welcome- Sharon Ann Walsh, President MSH
8:30 - 11:30 SCIENTIFIC SESSIONS
8:30 - 9:30 Basics for using Pro-EX-C in the Detection of High Grade
SC from Atypical Squamous Metaplasia
Lourdes Ylagen, Department of Pathology and Immunology, Division
of Surgical Pathology, Washington University School of Medicine, St.
Louis, Missouri
9:30 - 10:30 Safety in the Laboratory, What we take for granted.
Sylvia Casey
10:30-11:00 MSH Business Meeting (The meeting will be held in the
general session room immediately following the scientific session. All
are welcome to attend).
11:00 - 12:30 Exhibit Hall Open. (Ballpark Break)
WORKSHOPS 1:00 - 4:00
Workshop #4 Preparing for a CAP Inspection, Charlie Dorner,
Vision Biosystems
Workshop #5 Histologic Techniques in Hematopathology, Clinical
Applications and Troubleshooting, Sharad Mathur, M.D., VAMC, Kansas
City, Stacey Merica, HT(ASCP), VAMC, Kansas City
Workshop #6 Is your Processor Fighting You, Fight Back!
Pamela Marcum, University of PA,
School of Veterinary Medicine, Kennett Square, PA (Sponsored by Poly
Scientific)
All workshops are CEU approved.
Advance Registration must be postmarked by May 27th, 2006 (no refunds
after this date).
------------------------------
Message: 5
Date: Mon, 20 Mar 2006 15:08:30 -0500
From: "Delorise Williams" <DWilliams <@t> ciit.org>
Subject: [Histonet] North Carolina Spring Mtg.
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: Delorise Williams <dwilliams <@t> ciit.org>, david Weil
<dweil <@t> ciit.org>, Lisa Gates <lisa.d.gates <@t> gsk.com>,
joanna.c.barton <@t> gsk.com, Wanda Jones <wanpto <@t> aol.com>
Message-ID: <12816CB59E68184F9F5F28063E68B04702A60D85 <@t> xsrvr.ciit.org>
Content-Type: text/plain; charset="us-ascii"
The North Carolina Society of Histopathology Technologists will be
hosting their annual spring meeting at the Greensboro/ Highpoint Airport
Marriott in Greensboro, NC. on April 21-22, 2006. We welcome you to come
and experience North Carolina's beautiful springtime scenery. We have a
very interesting program planned with such presenters as Peggy Wenk,
Robert Lott, Lamar & Wanda Jones, Connie Wavrin and John Wilson from
Ventana. For more info, contact me at dwilliams <@t> ciit.org
Delorise Williams
CIIT Centers for Health Research
PO Box 12137
Research Triangle Park, NC 27709
(919) 558-1200
Voice Mail-(919) 558-1252
Fax-(919) 558-1300
------------------------------
Message: 6
Date: Mon, 20 Mar 2006 14:25:30 -0700
From: <Kathy.Johnston <@t> CLS.ab.ca>
Subject: RE: [Histonet] Re: mucicarmine
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4BC300747AF87A48BCDF8E48BC2885CE013652F2 <@t> mail1.calgary.com>
Content-Type: text/plain; charset="iso-8859-1"
We use the same stock here (Sigma) and dilute it 1:3 with dH2O. We do use
Harris hematoxylin instead though.
I have heard that you can use a tartrazine counterstain to emphasize the
mucin staining if that is something your pathologists prefer.
Kathy Johnston
Tech II Special Stains
Anatomic Pathology- DSC
Calgary Laboratory Services
770-3572
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Johnson,
Teri
Sent: March 20, 2006 11:48 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: mucicarmine
Dorothy,
It's been a while since I've done one, but I remember we got our best
results using Weigert's Iron Hematoxylin staining first, and then using
Sigma's mucicarmine stain. I don't quite remember if we used it
undiluted, but I think we did use it more concentrated than their
protocol called for. Where I trained, we made up the mucicarmine stain
and used undiluted stock to get brighter pink/rose staining results.
Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64133
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 7
Date: Mon, 20 Mar 2006 14:28:46 -0700
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] Human CD31, CD34, von Willebrands etc publication
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20060320140932.01b0a7a8 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
There has been a great deal of discussion about some of these markers and
this publication in J Histochemistry Cytochemistry just came out. If you
need a copy, I will happy to forward a pdf of the article privately.
Immunohistochemical Expression of Endothelial Markers CD31, CD34, von
Willebrand Factor, and Fli-1 in Normal Human Tissues
Marc P. Pusztaszeri, Walter Seelentag and Fred T. Bosman
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
------------------------------
Message: 8
Date: Mon, 20 Mar 2006 15:19:22 -0700
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] Rat CD8 marker attn: Patsy Ruegg
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20060320151602.01b0b040 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Patsy,
There is an excellent article (even though from 1995) on Rat CD8 (OX 8)
along with other rat CD markers and murine CD markers. The authors used
PLP as the fixative for paraffin embedded tissue.
Immunohistochemical detection of T cell subsets and other leukocytes in
paraffin embedded rat and mouse tissues with monoclonal antibodies. J
Histochem Cytochem 45(3):313-320,1995 Whiteland JL et al.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
------------------------------
Message: 9
Date: Tue, 21 Mar 2006 09:15:42 +0100
From: Anne-Sophie MARTINEZ <anne-sophie.martinez <@t> unicaen.fr>
Subject: [Histonet] (pas de sujet)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <441FB62E.5070000 <@t> unicaen.fr>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Many thanks for your answers!
ASM
------------------------------
Message: 10
Date: Tue, 21 Mar 2006 05:12:03 -0500
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: [Histonet] MVP II
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001701c64ccf$e6b52410$7e31d445 <@t> HPPav2>
Content-Type: text/plain; charset="us-ascii"
Have an old MVP II tissue processor, desperately needing several
alcohol/xylene containers. Leaking!
So if anyone (including vendors) has some old containers laying around, I'm
more than happy to pay for shipping.
Sorry vendors, I'm run a school, and have no money to buy a new or used
tisse processor. Would have a little money to buy the containers.
Will also entertain suggestions on a type of "glue" I could use to plug the
leaks, that resists alcohols and xylenes. That one's got me stumped!
Thanks.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
------------------------------
Message: 11
Date: Tue, 21 Mar 2006 14:13:35 +0100
From: Anne-Sophie MARTINEZ <anne-sophie.martinez <@t> unicaen.fr>
Subject: [Histonet] oysters larvae again
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <441FFBFF.2040707 <@t> unicaen.fr>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Hello,
(1) I plan to fix oysters larvae/juveniles (4 month) for in toto ISH.
Which fixative is the best : Davidson (10% formaldehyde 37-40%, 30%
ethanol 95%, 60% seawater, 10% acetic acid) or MEMPFA-T (0,1 M Mops pH
7,4; 2 mM EGTA; 1 mM MgSO4; 4% paraformaldehyde; 0,1% Tween 20)?
(2) Would it be possible to use the MEMPFA-T to fix and "decalcify" the
shelves of the juveniles for TEM (embedding in type lowyril, unicril,
LR-white resins) instead of classical fixative (4% PFA) and then EDTA?
Thank you very much again.
Anne-Sophie
------------------------------
Message: 12
Date: Tue, 21 Mar 2006 07:49:46 -0600
From: "Histonews" <dpconsult <@t> earthlink.net>
Subject: [Histonet] Illinois Society for Histotechnologists - Annual
Symposium May 18 & 19, 2006
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<!&!AAAAAAAAAAAYAAAAAAAAAKyrT9i9ZLBBkEjEHNFDzeHCgAAAEAAAAG9mxJm6FdJNpMd6IzCC
PxMBAAAAAA==@earthlink.net>
Content-Type: text/plain; charset="iso-8859-1"
Illinois Society for Histotechnologists
Annual Symposium Convention
May 18 & 19, 2006
Lisle Hilton, Lisle, IL
Histotechs Searching for the Tree of Knowledge
The Illinois Society for Histotechnologists invites you to join us for the
Annual Symposium/Convention which will be held this year at the beautiful
Hilton Lisle/Naperville on May 18 &19, 2006. Once again the 10 half-day
workshops will be presented along with one half day of lectures.
All workshops are approved for continuing education
Hilton Lisle/Naperville
3003 Corporate West Drive
Lisle, Illinois
630-505-0900
www.lislenaperville.hilton.com
Room rates for the 2006 ISH Symposium are $99 -$109
Please call by April 15 and mention the ISH when making your reservation to
receive this rate.
[The program and registration is included in this message below]
Any questions???
Eileen Dusek: e_dusek1 <@t> comcast.net (630) 527-3545
Judy Bordyn: jborydn <@t> comcast.net
Educational Programs
Thursday May 18
7:00 - 8:00 Registration [Rolls, coffee and juice provided]
Morning Lectures
8:00 - 8:15 President's Welcome Jane Chladny
8:15 - 9:00 Cytology for Histologists Michelle Benruebin
9:00 - 10:15 Future of Histology Jim Robinson
10:15 - 10:45 Break with Vendors
10:45 - 11:15 Transmissible Spongiform Encephalopathies:
A brief, Prospective from the Animal World
11:15 - 12:00 Histology Jeopardy Ray Ortiz
12:00 - 1:00 Lunch
1:00 - 4:30 Afternoon Workshops
[ 2:30 - 3:30 Break with Vendors]
#1 A Brave New World: An Introduction to Molecular Pathology.
Dr. Thomas Haas
#2 Dilutions, Titrations and then Some.
Jim Chalmers
#3 New Direction in the CAP Laboratory Accreditation Program
Dr. Francis E. Sharkey
Friday May 19
7:00 - 8:00 Registration
8:00 - 11:45 Morning Workshops
[ 9:45 - 10:30 Break with Vendors]
#4 DIY Basic Repair and Maintenance of the Equipment in Your Lab
Matt Mincer and Stanley Weglarz
#5 Florescence In Situ Hybridization (FISH),
Theory and Application on Paraffin Embedded Tissue
Yelina X. Noskina
#6 DAKO The Continuum from Antibodies to Personalized Medicine
Mary Cheles
11:45 - 1:00 Lunch & Awards Ceremony
#7 Emerging Infectious Diseases and Zoonotic Agents; the new, the old and
ugly
Maureen Doran and Jane Chladny
#8 How to Motivate your Team
Sally Johnson
#9 Histology Nuts and Bolts- Back to the Basics
MariAnn Mailhoit
WORKSHOP DETAILS
#1 The last several decades have shown an increase in the role of genetics
in a variety of laboratory testing methods. Diagnostic molecular pathology
has become one of the fastest growing fields in pathology, and promises to
continue to do so. This seminar will present basic genetic and molecular
principles that will allow continued familiarity with ongoing rapid progress
and advancement seen with this relatively new discipline. In Molecular
Pathology, molecular biology methods are used to test DNA and/or RNA from
patients specimens, diagnose disease, direct choice of therapy, detect
residual/recurrent disease after therapy and provide prognostic information
for the patient. The intent of this course will be to provide a foundation
in molecular pathology laboratory. Certain diseases will be presented as
case studies and illustration of methods used. Additionally, new areas of
research and development in molecular pathology will be examined.
#2. This is a presentation that takes the "student" back to some basic
laboratory practices. It will discuss what dilutions and titrations are and
how, when and why to set them up in a lab. Basic pipette use, calibration
and record keeping will be reviewed as well as different of pipettes.
Formulas for primary dilutions and making them from a working stock will be
discussed. Record keeping, "Spec Sheet" and control tissue will also be
presented. A cost comparison between concentrates and predilutes, showing
the pros and cons for each.
#3 This session will focus on changes to the CAP Laboratory Accreditation
process and inspection checklists. Topics will include an overview of a new
development in the accreditation program (e.g., unannounced inspections, new
Team Leader Checklists, Inspector tools and inspection techniques, focus on
patient safety, mandatory team leader training, and Anatomic Pathology
Checklists changes to Histology). The participants' will understand the
rationale behind these new developments
#4 Let's face it: Life in the lab is stressful enough without having to
worry whether your equipment will work when you get there, Over the years we
have found that many problems can be resolved quite
easily and with no technical background. This workshop is designed to help
the Histotech resolve many of these issues without having to "call the
service guys". We will attempt to show you as many quick and easy fixes as
we can. We will also discuss maintenance and some do' s and don'ts that will
help prevent future problems. Hopefully you find this information helpful
and use it to avoid potential needless service calls
#5 Although FISH has been a technique in use sine 1984 it was not commonly
available fro cytogenetic analysis until early 1990's, with the use of FISH
on paraffin embedded tissue sections first described in 1992. While the
principals of FISH technology are straightforward, proper technique is
important in obtaining accurate results. FISH technologies have become a
cornerstone in genetic research and the development of personalized drug
therapies. It is in the area of clinical assessment where FISH already
provides valuable information to physicians. Proper technique for the use of
FISH on paraffin sections, once established can be used for a myriad of
applications. One such FISH assay is the determination of HER2 status
currently provides valuable information regarding prognostic outcomes and
predicts therapeutic value. Overall, FISH ,a powerful tool in the area of
molecular and cytogenitic research, has expanded into the field of
Histology, where future assays may progress to the level that HER2 FISH
status holds today.
#6 Dako Targeted-therapy. Predictive medicine. What does all this mean and
where does the histology community fit in? The Analysis of a patient has
historically relied on morphology and the evaluation of individual
antibodies on pathological tissue. Therapeutic antibodies have become
important strategy in the treatment of various solid tumors requiring
involvement by pathology and laboratory medicine to meet the challenge. In
the future, personalized medicine will define the effects of a therapy based
on an individual's genes and protein profile. In this workshop we will
review this continuum leading us towards personalized medicine.
#7 What are the risks of handling biohazards such as Tuberculosis, herpes
B-virus and rabies. Discussion will include recommended procedures for
bioterrorism agents such as tularemia and anthrax, also emerging diseases,
like West Nile virus, SARS, avian flu and transmissible spongiform
encephalopathies. Handling of these and other biohazards will be covered in
this workshop. Exposure assessment, evaluation of risks and biosafety levels
will be addressed, as well as containment, engineering controls, personal
protective equipment and decontamination. Case histories of
zoonotic exposure in laboratory workers will be used to predict future
incident
#8. How to motivate your team is a dynamic and emergent seminar based on
research and practicality. It offers hands on strategy for communication and
ideas to enhance component to enjoy your job and be successful and
versatile. This seminar will also reveal the secrets on how to tap into your
teams talents and arrive at great quality with an improved turn around time
for your organization.
#9.Ever wonder why we do things the way we do them? As a participant you
will learn things like: What does formalin, alcohol , and xylene or xylene
substitute do to the tissue during processing? What is the proper way to
embed skin? Does it really matter how I turn the wheel on my microtome. H&E
stains are yucky, where and how do I resolve the problem? How thin ins too
thin and how thick is too thick. Whether you are a newcomer or an old
schooler, a review of basic Histology is essential.
INSTITUITIONAL PASS
2-Day Pass $300 _______
[Two techs from one institution may be present at any given time
during both days of the meeting] (Maximum of 8 different people)
Facility Information:
___________________________________________
___________________________________________
___________________________________________
Thursday May 18
Morning Session, LECTURE
Tech #1_________________________________
Tech #2_________________________________
Afternoon Session, WORKSHOP
Tech #1_________________________________
Tech #2_________________________________
Friday, May 19
Morning Session, WORKSHOP
Tech #1_________________________________
Tech #2_________________________________
Afternoon Session, WORKSHOP
Tech #1_________________________________
Tech #2_________________________________
1 Day Pass $175_________
[Two Techs from one institution may be present at any given time
on Thursday OR Friday] (Maximum of 4 different people)
Facility Information
__________________________________________________
__________________________________________________
___________________________________________________
Please choose day techs will be attending:
__________May 18
__________May 19
Morning Session List Lecture or Workshop
Tech #1__________________________________________
Tech #2 _________________________________________
Afternoon Session, List Workshop
Tech #1__________________________________________
Tech #2__________________________________________
2006 ISH SYMPOSIUM / CONVENTION REGISTRATION FORM
Please return by April15, 2006
$30_______ Registration Fee (non refundable)___Non Members [Includes ISH
Membership]
$10_______ Registration Fee (non refundable)___Members
Thursday May 18
$45_______ Lecture Session (Morning)
Afternoon Workshops (Choose 1)
$45_______#1 Brave New World, Molecular Pathology
$45_______#2 Dilution, Titration and Then Some
$45_______#3 New Direction in the CAP Lab. Accreditation
Friday May 19
Morning Workshops (Choose 1)
$45_______#4 DIY Repair and Maintenance of Equipment
$45_______#5 FISH on Paraffin Embedded Tissue
$45_______#6 Continuum from Antibody to Personalized Medicine
Afternoon Workshops (Choose 1)
$45_______#7 Emerging Infectious Diseases
$45_______#8 How to Motivate your Team
$45_______#9 Histology Nuts and Bolts
LUNCH IS INCLUDED BOTH DAYS TO ALL ATTENDEES
Name________________________________________________________
Home Address:
________________________________________________
_________________________________________________
Home Phone_______________________
Work Address:
_________________________________________________
_________________________________________________
Work Phone _______________________
Would you prefer information sent to home or work?_______________
eMail Address _____________________________________________________
I would prefer to be contacted primarily by email. YES_____NO______
All information will be used only for ISH membership purposes
Please return by April 15, with check payable to:
Illinois Society for Histotechnologists
C/O Judy Bordyn
532 65th Street
Downers Grove, Ill 60516
Any questions???
E-mail Judy at jborydn <@t> comcast.net or
Eileen Dusek at e_dusek1 <@t> comcast.net (630) 527-3545
------------------------------
Message: 13
Date: Tue, 21 Mar 2006 05:56:40 -0800 (PST)
From: Justin Thomas <jstn192 <@t> yahoo.com>
Subject: [Histonet] RE: Cheap Accessories
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20060321135640.40293.qmail <@t> web35712.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I am head of histology/pathology at a hospital in California, and I am
putting the word out for members to respond....there is a company that can
make identical microwave/any other accessories made of hard or soft material
to accommodate equipment relatively economical! I have ordered a number of
different accessories from this company, and they have great service. I
think each member will be pleased with their services. Just reply to my
email if you are interested....and i will give you their contact information
!
-Thanks
---------------------------------
Relax. Yahoo! Mail virus scanning helps detect nasty viruses!
------------------------------
Message: 14
Date: Tue, 21 Mar 2006 08:20:08 -0600
From: "Orr, Rebecca" <ROrr <@t> enh.org>
Subject: [Histonet] desperately seeking Chewie
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A7FC3A4F98964A44B0B71B7B25EA6F96055B7149 <@t> EXCHANGE03.enhnet.org>
Content-Type: text/plain; charset="US-ASCII"
I'm looking for Chewie from Yuma Regional.
I evidently wrote your email address wrong and can't get that
information through to you.
It keeps bouncing back
Let me know!
Bec
Becky Orr CLA,HT(ASCP)
IHC Lead
Evanston Northwestern Healthcare
847-570-2771
------------------------------
Message: 15
Date: Tue, 21 Mar 2006 08:43:45 -0600
From: "Charles Scouten" <cwscouten <@t> myneurolab.com>
Subject: [Histonet] Looking for Richard Thrift
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5784D843593D874C93E9BADCB87342AB01306C41 <@t> tpiserver03.Coretech-holdings.com>
Content-Type: text/plain; charset="us-ascii"
I have an old email address for Richard Thrift and need to contact him.
Anybody know where he is now?
Cordially,
Charles W. Scouten, Ph.D.
myNeuroLab.com
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300 x 342
FAX 314 522 0377
cwscouten <@t> myneurolab.com <mailto:cwscouten <@t> myneruolab.com>
http://www.myneurolab.com
------------------------------
Message: 16
Date: Thu, 9 Mar 2006 11:24:15 -0500
From: "Atkin, Thelda J LTC WRAIR Wash-DC" <Thelda.Atkin <@t> US.ARMY.MIL>
Subject: [Histonet] Histotechnician Position announcement Walter Reed
Army Institute of Research- Comparative Pathology
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C389E03698DBCE49990D6BF1AA376602E6A7E0 <@t> AMEDMLNARMC135.amed.ds.army.mil>
Content-Type: text/plain; charset="iso-8859-1"
DEPARTMENT OF THE ARMY
Vacancy Announcement Number: NEBB06178220
Opening Date: March 10, 2006 Closing Date: March 20, 2006
Position: HISTOPATHOLOGY TECHNICIAN, DE-0646-3
Salary: $44,856 - $70,558 Annual
Place of Work: WALTER REED ARMY INSTITUTE OF RESEARCH, DIVISION OF
PATHOLOGY, DEPARTMENT OF COMPARATIVE PATHOLOGY, SILVER SPRING, MD
Position Status: This is a Permanent position. -- Full Time
Number of Vacancy: 01
Duties: Work involves knowledge of all histopathology procedures to ensure
quality and promptness. Must provide expert supervision and technical skills
to produce tissue samples suitable for microscopic examination by
pathologists and other investigators. The work can be very tedious and
requires significant patience to produce high quality stained specimens of
2-20 micron thickness. Candidate is expected to perform unusual techniques,
such as frozen microtomy, immunohistochemistry and plastic
embedding/microtomy. Work involves leading three or more employees in
accomplishing their work. Must have the ability to assist a team through
knowledge and application of leadership and team building skills and
techniques such as group facilitation, coordination, coaching, problem
solving, interpersonal communication, and integration of work processes and
products.
About the Position: This position is for a Lead Histotechnologist who will
be responsible for all aspects of the operation and management
of the Department of Comparative Pathologys Histology Laboratory. The lab
produces an average of 16,000 slides
and 10,000 paraffin blocks a year in support of the Walter
Reed Army Institute of Research and Navy Research Medical
Command experimental protocols and the Division of Veterinary
Medicines quality assurance program.
Who May Apply:
<https://cpolwapp.belvoir.army.mil/public/forward/VAB?id=whoMayApply>
(Click on Who May Apply)
* Veterans eligible under Veterans Employment Opportunities Act of
1998. (VEOA)
* Interagency Career Transition Assistance Plan (ICTAP) eligibles.
* Defense Civilian Intelligence Personnel System (DCIPS) eligibles.
* All Federal employees serving on a career or career-conditional
appointment.
* Department of Defense employees serving on a Career or Career
Conditional Appointment.
* Current Army employees with competitive status (includes Army
employees serving on a career or career-conditional appointment).
* Reinstatement eligibles.
Qualifications: Click on link below to view qualification standard.
General Schedule
<https://cpolwapp.belvoir.army.mil/public/forward/VAB?id=qualifications&sele
ction1=occGroup7>
* 1. The Walter Reed Army Institute of Research is participating in an
alternative personnel system known as the Personnel Demonstration Project.
This position incorporates the GS-09 to GS-11 level. In keeping with the
Demonstration pay fixing policies, employees already in the band will not
receive a pay increase. 2. SPECIALIZED EXPERIENCE: Candidates for this
position must show in their resume that they have one year of specialized
experience and training that provided knowledge of histopathology procedures
to ensure quality and promptness. This year of specialized experience must
have included responsibility for performing unusual techniques, such as
frozen microtomy, immunohistochemistry and plastic embedding / microtomy.
* GS-06 and above: One year of experience directly related to this
occupation equivalent to the next lower grade. Graduate education or an
internship meets the experience required when it is directly related to the
work of the position.
* The experience described in your resume will be evaluated and
screened for the Office of Personnel Management's basic qualifications
requirements, and the skills needed to perform the duties of this position
as described in this vacancy announcement.
* Foreign education must be evaluated for U.S. equivalency in order to
be considered for this position. Please include this information in your
resume.
* One year of experience in the same or similar work equivalent to at
least the next lower grade or level requiring application of the knowledge,
skills, and abilities of the position being filled.
* Only degrees from an accredited college or university recognized by
the Department of Education are acceptable to meet positive education
requirements or to substitute education for experience. For additional
information, please go to the Office of Personnel Management (OPM) and U.S.
Department of Education websites at - http://www.opm.gov/qualifications and
http://www.ed.gov/admins/finaid/accred/index.html
Other Information:
<https://cpolwapp.belvoir.army.mil/public/forward/VAB?id=otherInformation>
(Click on Other Information)
* The Department of Defense (DoD) policy on employment of annuitants
issued March 18, 2004 will be used in determining eligibility of annuitants.
The DoD policy is available on
<http://www.cpms.osd.mil/fas/staffing/pdf/rem_ann.pdf>
http://www.cpms.osd.mil/fas/staffing/pdf/rem_ann.pdf
* Permanent Change of Station (PCS) expenses are not authorized.
* Temporary Duty (TDY) travel is 10 percent.
Other Advantages: The Walter Reed Army Institute of Research is 10 minutes
from Washington, D.C. If you would like more information
about our installation, please visit our website at
http://wrair-www.army.mil
Other Requirements:
<https://cpolwapp.belvoir.army.mil/public/forward/VAB?id=otherRequirements>
(Click on Other Requirements)
* Must be able to obtain and maintain a Secret security clearance.
* A medical examination is required.
* You will be required to provide proof of U.S. Citizenship.
* Extended probationary period for the Engineers and Scientists
Occupational Family will be three years. Extended probationary period for
all other Occupational Families will be two years.
* Male applicants born after December 31, 1959 must complete a
Pre-Employment Certification Statement for Selective Service Registration.
* Direct Deposit of Pay is Required.
* This is a DOD Demonstration Project position.
How to Apply:
<https://cpolwapp.belvoir.army.mil/public/forward/VAB?id=applyInstruction&se
lDoc=Resumix> (Click on How to Apply)
* Resumes must be received by the closing date of this announcement.
* Self-nomination must be submitted by the closing date.
* Resume must be on file in our centralized database.
* Announcements close at 12:00am (midnight) Eastern Time.
If your resume is currently in our central database, you may click here to
Self Nominate
<https://cpolwapp.belvoir.army.mil/public/forward/VAB?id=selfNom&annouNum=NE
BB06178220&position=HISTOPATHOLOGY TECHNICIAN,
DE-0646-3&payPlan=DE&series=0646&grade=3>
Click here to use the Army Resume Builder
<https://cpolwapp.belvoir.army.mil/public/forward/VAB?id=rb> to create your
resume. Follow the instructions in this vacancy announcement to apply for
the job.
Point of Contact: Central Resume Processing Center, 410-306-0137,
applicanthelp <@t> cpsrxtp.belvoir.army.mil
<https://cpolwapp.belvoir.army.mil/public/forward/VAB?id=EOE> THE
DEPARTMENT OF DEFENSE IS AN EQUAL OPPORTUNITY EMPLOYER
------------------------------
Message: 17
Date: Tue, 21 Mar 2006 06:52:25 -0800 (PST)
From: beth zink <beth_zink <@t> yahoo.com>
Subject: [Histonet] hello
To: histonet <@t> pathology.swmed.edu
Message-ID: <20060321145225.2646.qmail <@t> web60123.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Could you please send me directions on how to ask questions and get
responses?
Thanks
Beth
---------------------------------
Yahoo! Mail
Bring photos to life! New PhotoMail makes sharing a breeze.
------------------------------
Message: 18
Date: Tue, 21 Mar 2006 07:35:50 -0800
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Subject: [Histonet] Job posting
To: histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <C0455D56.B8F7%ploykasek <@t> phenopath.com>
Content-Type: text/plain; charset="ISO-8859-1"
I'm posting this job opening for a friend in research who is having trouble
with her internet connection. This position is located north of Seattle.
HISTOLOGIST
MDS Pharma Services, Division of Efficacy-Pharmacology, is a
therapeutically focused contract research organization that specializes in
bone and central nervous system (CNS) biologies. We are currently seeking an
experienced histologist to primarily participate in the Company¹s
pre-clinical bone research programs. Responsibilities will include hard
tissue processing, embedding, thin sectioning, ground sections (using the
EXAKT system), staining of both thin and ground sections, and the
development and validation of laboratory techniques in support of
client-sponsored research programs. Specific experience in orthopedic
histological techniques is highly desired. Qualified applicants will possess
a BS/MS degree and/or HT/HTL certification, 4+ years of related histology
experience and documented expertise in hard tissue preparation and
sectioning (usually MMA), and use of the EXAKT system. Candidates with prior
experience working in a GLP compliant laboratory and expertise with
histomorphometry will be considered superior.
MDS Pharma Services offers an energetic and challenging research environment
with a competitive compensation and benefits package including equity
participation. Interested applicants can apply via the MDS Pharma Services
website located at www.mdsps.com <http://www.mdsps.com/> . The position can
be found by searching for jobs in the United States, and location,
Bothell-West.
Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA
-------------------------------------------------------------------------
This e-mail message, including any attachments, is for the sole use of
the intended recipients and may contain privileged information. Any
unauthorized review, use, disclosure or distribution is prohibited. If
you are not the intended recipient, please contact the sender by e-mail
and destroy all copies of the original message, or you may call PhenoPath
Laboratories, Seattle, WA U.S.A. at (206) 374-9000.
------------------------------
Message: 19
Date: Tue, 21 Mar 2006 09:38:51 -0600
From: "Charles.Embrey" <Charles.Embrey <@t> carle.com>
Subject: RE: [Histonet] hello
To: "beth zink" <beth_zink <@t> yahoo.com>
Cc: Histonet <@t> pathology.swmed.edu
Message-ID:
<C320919A1C432845938BCD8CC3294A3002A7CDF1 <@t> EXCHANGEBE1.carle.com>
Content-Type: text/plain; charset="us-ascii"
You just did. That is all there is to it.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of beth
zink
Sent: Tuesday, March 21, 2006 8:52 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] hello
Could you please send me directions on how to ask questions and get
responses?
Thanks
Beth
---------------------------------
Yahoo! Mail
Bring photos to life! New PhotoMail makes sharing a breeze.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 20
Date: Tue, 21 Mar 2006 11:23:04 -0500
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: RE: [Histonet] oysters larvae again
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<09C945920A6B654199F7A58A1D7D1FDE017176A4 <@t> lsexch.lsmaster.lifespan.org>
Content-Type: text/plain; charset="iso-8859-1"
I don't have a background in ISH, so I can't offer an opinion on the best
fixative for that purpose.
Regarding your second question, while I'm not familiar with either fixative,
I can see that the Davidson fixative, containing 10% acetic acid and no
buffers, would decalcify (dissolve) larval oyster shells. But the MEMPFA-T
appears to be made up in pH 7.4 buffer, in which case I don't see how it
would decalcify. In any case, I am wondering why you are using a
formaldehyde-based fixative for TEM? Ordinarily a glutaraldehyde-based
fixative would be preferable for TEM.
> ----------
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> Anne-Sophie MARTINEZ
> Sent: Tuesday, March 21, 2006 5:13 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] oysters larvae again
>
> Hello,
> (1) I plan to fix oysters larvae/juveniles (4 month) for in toto ISH.
> Which fixative is the best : Davidson (10% formaldehyde 37-40%, 30%
> ethanol 95%, 60% seawater, 10% acetic acid) or MEMPFA-T (0,1 M Mops pH
> 7,4; 2 mM EGTA; 1 mM MgSO4; 4% paraformaldehyde; 0,1% Tween 20)?
> (2) Would it be possible to use the MEMPFA-T to fix and "decalcify" the
> shelves of the juveniles for TEM (embedding in type lowyril, unicril,
> LR-white resins) instead of classical fixative (4% PFA) and then EDTA?
> Thank you very much again.
> Anne-Sophie
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 21
Date: Tue, 21 Mar 2006 17:23:48 +0100
From: b003046 <@t> nf.au.dk
Subject: [Histonet] Lineage Negative Cells
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1142958228.44202894e08a0 <@t> webmail.nf.au.dk>
Content-Type: text/plain; charset=ISO-8859-1
Hi,
I am looking for a method to exclude lineage negative cells (Lin-) in rats
in
immunohistochemistry (paraffin-embedded tissue). Which antibodies should I
use
and is it possible to buy a detection kit? Any help would be appreciated.
kind regards
Mette K. Hagensen
------------------------------
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 28, Issue 33
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