[Histonet] Re: mucicarmine

[Kathy.Johnston <@t> CLS.ab.ca]

We use the same stock here (Sigma) and dilute it 1:3 with dH2O. We do use Harris hematoxylin instead though.

I have heard that you can use a tartrazine counterstain to emphasize the mucin staining if that is something your pathologists prefer.

Kathy Johnston
Tech II Special Stains
Anatomic Pathology- DSC
Calgary Laboratory Services
770-3572


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Johnson,
Teri
Sent: March 20, 2006 11:48 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: mucicarmine


Dorothy,

It's been a while since I've done one, but I remember we got our best
results using Weigert's Iron Hematoxylin staining first, and then using
Sigma's mucicarmine stain. I don't quite remember if we used it
undiluted, but I think we did use it more concentrated than their
protocol called for. Where I trained, we made up the mucicarmine stain
and used undiluted stock to get brighter pink/rose staining results.

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64133

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