[Histonet] Re: mucicarmine

Johnson, Teri TJJ <@t> Stowers-Institute.org
Mon Mar 20 12:47:51 CST 2006


It's been a while since I've done one, but I remember we got our best
results using Weigert's Iron Hematoxylin staining first, and then using
Sigma's mucicarmine stain. I don't quite remember if we used it
undiluted, but I think we did use it more concentrated than their
protocol called for. Where I trained, we made up the mucicarmine stain
and used undiluted stock to get brighter pink/rose staining results.

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64133

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