[Histonet] lectin stains and antigen retrival

[Gayle Callis]

Yes, lectins are stable although trypsin enzyme digestion at 37C may be 
better than citrate buffer retrieval.  Also, there are buffer 
considerations for staining, i.e. TRIS Lectin buffer with Mg and Ca 
added.  The book gives the recipe for this and with some lectins PBS should 
be avoided and also normal serums.

AND there is an excellent book on Lectin Histochemistry , a concise 
practical handbook SA Brooks et al, ISBN#1859961002  that gives IHC 
staining methods, etc.  It is not expensive and Springer Verlag is the 
publisher source.



01:18 AM 3/19/2006, you wrote:
>Dear Histonetters,
>does anybody know if lectin stains are stable after the tissue
>section (20u, paraffin embedded) was subjected to heat antigen
>retrieval in citric acid pH 6.0 ?
>Thanks for your help in advance!
>Cheers
>franzef
>
>Dr. med. Franz-Josef Müller
>Zentrum für Integrative Psychiatrie, Kiel
>Zellbiologisches Labor
>Niemannsweg 147
>24105 Kiel
>GERMANY
>
>Tel.: +49 431 597 4169
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)