Rene J Buesa
rjbuesa <@t> yahoo.com
Mon Mar 20 10:19:02 CST 2006
Are you using the left-over of the not so frequently used staining kit?
How old are the solutions?
I always stained nuclei first, followed by the mucicarmin sol. (35 secs. boost in the MW oven) followed by 1 hour at 60ºC in the water bath.
From that moment on everything should be done as quickly as possible:
rinse in water, quick rinse in 95% ethanol; quickly (only a few seconds) in a strong (2% alc.) fast green solution; followed by a very fast dehydration/clearing (only 1-2 dips in the alcohols).
Hope this will help you.
"Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com> wrote:
We have been doing our mucicarmine on the Nexus special stainer and due
to the fact that we do not do a lot of these, I decided to bring it back
to handstaining. I am using the Southgate's modification of Mayer's
Technique and having a very hard time getting the stain to look nice and
sharp! Does anyone have any procedures they use or suggestions as to
what could be our problem? Our muci is not staining the bright red and
even the nuclei are not stained very dark. We have tried various times
and strengths of the mucicarmine and even from two different companies!
Help and thanks for any and all suggestions!
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