[Histonet] MT's and HT's

Mon Mar 20 10:11:28 CST 2006

Thank you to each and everyone who replied to me privately on the Issue of HT's and MT's as managers.
It turned from a warm topic to a very HOT topic.
Someone asked for some feedback, because a lot of the respondents did not want to publicize their opinions.  I'll be very general here.

Most of the opinion is that all the MT's that are managers were trained and had a propensity for Histology work. Most took pay cuts to work as histo techs.  Most commented that the better salaries arrived with management positions. I have received comments from many saying MT's cannot perform with the same skills as an HT.  I also received the exact opposite, that they are MORE capable!  

There is varying opinion about the COMPLEXITY of testing in the histology lab.  There is also varying opinion about critical thinking skills that are learned in a formal setting(university) and those same  skills developed specifically on the job (HT with 10 years experience.)

The  pay in the histo lab seems to be lower because the complexity of testing is presumed to be less.

That's the feedback, not necessarily my opinion.  Thanks again to everyone.  I've gotten the feedback I needed. 

Becky

 Becky Orr CLA,HT(ASCP)
IHC Lead 
Evanston Northwestern Healthcare
847-570-2771

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Subject: Histonet Digest, Vol 28, Issue 31

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Today's Topics:

   1. CD4 & CD8 by Immunohistochemistry (Marilyn Johnson)
   2. lectin stains and antigen retrival (Franz-Josef M?ller)
   3. Re: lectin stains and antigen retrival (Rene J Buesa)
   4. Florida Society For Histotechnology Annual Meeting
      (Santiago, Jerry)
   5. cd8 on rats (pruegg <@t> ihctech.net)

----------------------------------------------------------------------

Message: 1
Date: Sat, 18 Mar 2006 17:17:58 -0700
From: "Marilyn Johnson" <marjoh3 <@t> telus.net>
Subject: [Histonet] CD4 & CD8 by Immunohistochemistry
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <004401c64aea$93c4d1b0$6501a8c0 <@t> VALUED20606295>
Content-Type: text/plain;	charset="iso-8859-1"

Hi Histonetters,
In approximately two months, our Lab. will be initiating a new project using skin sections of mice.
We will be using IHC to demonstrate infiltrates at vaccine sites including CD4, CD8, B-cells, 
monocytes, macrophages, etc. 
Any assistance would be greatly appreciated.
Thank you in advance. 

Marilyn Johnson
Alberta Agriculture
Edmonton, Alberta, Canada

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Message: 2
Date: Sun, 19 Mar 2006 09:18:20 +0100
From: Franz-Josef M?ller <franzef <@t> gmx.de>
Subject: [Histonet] lectin stains and antigen retrival
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <E1E746AF-4BDD-496F-9874-2EBF80706FCE <@t> gmx.de>
Content-Type: text/plain;	charset=ISO-8859-1;	delsp=yes;	format=flowed

Dear Histonetters,
does anybody know if lectin stains are stable after the tissue  
section (20u, paraffin embedded) was subjected to heat antigen  
retrieval in citric acid pH 6.0 ?
Thanks for your help in advance!
Cheers
franzef

Dr. med. Franz-Josef Müller
Zentrum für Integrative Psychiatrie, Kiel
Zellbiologisches Labor
Niemannsweg 147
24105 Kiel
GERMANY

Tel.: +49 431 597 4169

------------------------------

Message: 3
Date: Sun, 19 Mar 2006 07:35:56 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] lectin stains and antigen retrival
To: Franz-Josef "Müller" <franzef <@t> gmx.de>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <20060319153557.93612.qmail <@t> web61214.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Franz-Josef:
  Lectins, as you know, will react with carbohydrates in the tissue. The lectin will attach to the carbohydrates, and the anti-lectin-antibody will bind to the lectin identifying the carbohydrates sites in a 2 steps (indirect) reaction.
  Since carhohydrates are not affected with formalin fixation as proteins do, it is not necessary to subject the sections to antigen retrieval (HIER) this being a step you can avoid if just trying to use the lectins.
  They should be stable after HIER since some carbohydrate detecting techniques (like PAS) are done in acid pH solutions.
  Hope this will help!
  René J.

Franz-Josef Müller <franzef <@t> gmx.de> wrote:
  Dear Histonetters,
does anybody know if lectin stains are stable after the tissue 
section (20u, paraffin embedded) was subjected to heat antigen 
retrieval in citric acid pH 6.0 ?
Thanks for your help in advance!
Cheers
franzef

Dr. med. Franz-Josef Müller
Zentrum für Integrative Psychiatrie, Kiel
Zellbiologisches Labor
Niemannsweg 147
24105 Kiel
GERMANY

Tel.: +49 431 597 4169

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Message: 4
Date: Sun, 19 Mar 2006 11:23:43 -0500
From: "Santiago, Jerry" <jerry.santiago <@t> jax.ufl.edu>
Subject: [Histonet] Florida Society For Histotechnology Annual Meeting
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<D8D959F60FE58F4EB342F7694D7EFE86291A96 <@t> jaxmail.umc.ufl.edu>
Content-Type: text/plain;	charset="iso-8859-1"

The Florida Society for Histotechnology is preparing for their Annual Symposium May 5 - 7, 2006 Embassy Suites Deerfield Beach Florida.  All Information regarding registration, hotel and exhibiton can be accessed through our website at www.fshgroup.org.  Online registration is now available.We will be offering 16 workshops and the exhibit will consist of approximately 40 exhibitors.

Exhibitors can download an exhibiton form from the website and mail to the address printed. 

Remember this is a licensure renewal year and all your mandatory's will be offered during this symposium.

Any questions please feel free to contact Susan Clark at 954-987-2020 X 5371

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Message: 5
Date: Sun, 19 Mar 2006 10:33:42 -0700
From: pruegg <@t> ihctech.net
Subject: [Histonet] cd8 on rats
To: <histonet <@t> pathology.swmed.edu>
Message-ID: <200603191733.k2JHXh46094702 <@t> pro12.abac.com>
Content-Type: text/plain;	charset="us-ascii"

OK, I think I have some good advise on doing CD8 on frozen rat tissue, now
has anyone gotten CD8 to work of ffpe rat tissue yet.  I know, I know, this
question comes up all the time for mice and I have been given procedures
that I struggled over with no success, but I was hoping it might be more
promising for rats.

Patsy

BTW

Those signed up to the IHCRG list serve.  We are switching host servers so
be patient while we get back up to speed.

Patsy Ruegg, HT(ASCP)QIHC

IHCtech, LLC

Fitzsimmons BioScience Park

12635 Montview Blvd. Suite 215

Aurora, CO 80010

P-720-859-4060

F-720-859-4110

wk email pruegg <@t> ihctech.net

website www.ihctech.net

------------------------------

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