[Histonet] RE: Sudan Black Staining

Kellar, Eric C Eric.C.Kellar <@t> questdiagnostics.com
Fri Mar 17 09:24:16 CST 2006

To slow Sudan Black B (a nonionic, hydrophobic dye) 'bleeding' on unfixed cryostat sections, try running a duplicate slide and compare with this method:

1.	Air dry duplicate slide after staining.
2.	Clear slide through 3 changes of fresh Xylene or Xylene Substitute.
3.	Glass coverslip using a permanent mounting media.

The synthetic mountant will eventually dissolve some of the dye-lipid complex, but it will give you a longer window of time to microscopically examine your slides.

Eric C. Kellar
Quest Diagnostics Inc. Miami

I am working on the Sudan Black stain.  Do the slides need to be read 
immediately due to bleeding of the stain after approximately 24 hours?

Thanks for any information you can give me about the stain.
Sharon Willman

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