[Histonet] RE: Histonet Digest, Vol 28, Issue 27

[Orr, Rebecca]

Stacy,
I wrote a sentence describing the issues of background staining.  I then
described what detection kits we use in our lab. We use both
polymer(Dako/Biocare)  and the Ventana I view on the benchmark.  I also
added instructions to determine if Endogenous Biotin is evident
(omitting primary and secondary and just adding the label and chromogen
on one slide, just the chromogen on a second slide...) 
I put something in there about following manufacturer's recommendations
on the data sheets. I addressed protein blocks and endogenous peroxidase
all in the same section and called it "Blocking Reagents".
I was just inspected in February and the inspectors and I  scrutinized
that regulation especially since it was new.  I passed so I'm thinking
what I wrote was adequate.  I'll go ahead and send you the excerpt with
my references in a separate file.  Let me know if you can't receive
files from strangers.  I can fax if you'd like
Have a great weekend
Becky

 Becky Orr CLA,HT(ASCP)
IHC Lead 
Evanston Northwestern Healthcare
847-570-2771
 

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Message: 6
Date: Thu, 16 Mar 2006 14:01:46 -0500
From: "Stacy McLaughlin" <Stacy_McLaughlin <@t> cooley-dickinson.org>
Subject: [Histonet] Endogenous Biotin and CAP regulations
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	
<CB8866A9D4ECB049968DB571CF4863A301D3017E <@t> cdhmail01.cooley-dickinson.org
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 Would anyone be willing to share how they address this issue, regarding
    CAP reg: ANP.22615 "If the laboratory uses an avidin-biotin complex
    (ABC) detection system (or a related system such as
streptavidin-biotin
    or neutravidin-biotin), is there a policy that addresses nonspecific
    false positive staining from endogenous biotin?
    We're currently using the Ventana Nexes, with biotin-streptavidin
    detection system. 
    Are there specific vendors your recommend for these reagents?
    Your answers are appreciated!
    Stacy
    Stacy McLaughlin HT (ASCP)
    Lead Tech, Histology
    Coolely Dickinson Hospital



Stacy McLaughlin HT (ASCP)
Lead Tech, Histology