[Histonet] Double stain ?

[Emerson, Rachael]

Hello. I am attempting to do a sequential double stain with 2 different
antibodies.
Unfortunately, one requires using Triton-X 100 for antigen retrieval and the
other requires using trypsin.
I've run trials using several different antigen retrieval methods and these
are the only ones that work.
I'm afraid that performing 2 different retrieval steps will cause the stain
not to work. I would really appreciate any thoughts or suggestions.
I am working with paraformaldehyde fixed mouse embryos.


Thanks 
Rachael Emerson