[Histonet] question

[Kemlo Rogerson]

Lipid Autoradiography may be the answer; label the removed fat with an
isotope (tritium is favoured as its beta particle track is about 1.5 u) and
then redetect with a nuclear track emulsion. I suppose you stain a 'control'
slide with a fat stain (Oil red O or Sudan Black) then superimpose the
results of the autoradiography.

If you are interested then I can give you the appropriate references.

Kemlo Rogerson
Pathology Manager
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Mob 07749 754194
 

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-----Original Message-----
From: Kobler, James [mailto:JKOBLER <@t> PARTNERS.ORG] 
Sent: Wednesday, March 15, 2006 6:56 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] question

I'm soliciting advice about the best ways to examine fat tissue
histologically.
We are preparing fat tissue for autografting (reinjection through a needle)
and
want to look at damage to the fat tissue caused by extrusion through a
needle.
Subcutaneous fat is surgically excised, scraped to remove most of the
fibrous
component, stuffed into a syringe and then extruded out through a fine
needle
under considerable pressure.  I would appreciate any suggestions about the
best
ways to compare normal fat tissue with the extruded fat 'goop'. In
particular we
would like to be able to quantify the number of viable cells.  Thanks, Jim
Kobler, Ph.D., Dept. of Surgery, Harvard Medical School

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