[Histonet] Mayer's Hematoxylin

[Jennifer MacDonald]

   Karen,

   Our  students  are  using Hematoxylin 1, by Richard   staining  by hand, so there is great variation of tec   students,  but  very  little  in  the  staining of the nuclei.&nb   Richard-Allan  has  a great chart for optimizing the H&E with their r   eagents.

   Jennifer MacDonald





   -----histonet-bounces <@t> lists.utsouthweste
     To: <histonet <@t> lists.utsouthwestern.edu>
     From: "Bauer,     Sent by: histonet-bounces <@t> lists.uts     Date: 03/15/2006 09:02AM
     Subject: [Histonet] Mayer's      Hello to all,
     I'm  looking  for  a Pre-Made Mayer's H     for  our  routine  H&E  staining  procedure. &nbs     Mayer's  Hematoxylin  from  scratch, but have recently been g     permission  to buy something that is pre-made.  We've tried Harris     ,  but  the doctors do not like the differentiation that take place
     with  usin     tech's  dip migh     the  differentiator.) &nbs     staining, no matter how careful we      I'm looking for something that stains ti     does not need differentiation.  Everything t     is  not  working  out to their satisfaction.  We us     for  our  FS  set  up,  and  they do not want that either (for the     routine H&E's).
     Does  anyone have any ideas or tips for me? &nbs     appreciative.
     Thanks in advance,
     Karen Baue     Histology Supervisor
     Luther Hospital
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