Mouse intestine in [Histonet] Bouin Solution

Gayle Callis gcallis <@t>
Wed Mar 15 13:39:07 CST 2006

We would fix it no more than overnight although 72 hours is permissable, 
transfer to 70% and do a couple of changes in this alcohol to help remove 
picric acid.   Hopefully you are rinsing the fecal contents out before 
doing this, we would actually then fill the intestine with Bouins using a 
syringe.  Messy but really fixes villi nicely.

At 11:06 AM 3/15/2006, you wrote:

>Hi Histonetters,
>       Can someone please tell me what is the standard Bouin fixation time
>for mouse small intestine.
>Thanks.   Helen  (hej01 <@t>
>Histonet mailing list
>Histonet <@t>

Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717

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