[Histonet] Mayer's Hematoxylin

[Patti Loykasek]

Karen, our research department uses Mayer's hematolxylin from BBC. They use
it as an IHC counter stain, and it works well. We have not used it for H&E.
The phone # for BBC is 360-629-4477.

Patti Loykasek


 Hello to all,
> 
> I'm looking for a Pre-Made Mayer's Hematoxylin that we can use for our routine
> H&E staining procedure.  We make up our Mayer's Hematoxylin from scratch, but
> have recently been given permission to buy something that is pre-made.  We've
> tried Harris, but the doctors do not like the differentiation that take place
> with using that kind of hematoxylin.  (We stain by hand, so one tech's dip
> might be longer or shorter than another tech's dip in the differentiator.)
> They are seeing variations in nuclear staining, no matter how careful we are
> with our "dips". 
> 
> I'm looking for something that stains tissues in 5-10 minutes and does not
> need differentiation.  Everything that I've tried so far is not working out to
> their satisfaction.  We use a Gill's III for our FS set up, and they do not
> want that either (for the routine H&E's).
> 
> Does anyone have any ideas or tips for me?  I'll be greatly appreciative.
> 
> Thanks in advance,
> 
> Karen Bauer HT(ASCP)
> Histology Supervisor
> Luther Hospital
> Eau Claire,  WI
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