[Histonet] alkaline phosphatase in cartilage

[Tamara Franz-Odendaal]

I am looking for a protocol to do Alkaline phosphatase staining of cartilage in 2cm fish by whole-mount.   I have a protocol for sections and am wondering how much longer I need to stain to be sure that staining has penetrated everywhere and also whether I need to replace the solution every few hours.  Anyone have any insights?

Thanks
Tamara

Dr. Tamara Franz-Odendaal
Post-doctoral fellow
Biology Dept., Dalhousie University
Halifax, NS, B3H 4J1
Canada