[Histonet] how to unfold section after mounting?

[IGOR NASONKIN]

dear histonetters,

Does anyone have experience trying to unfold frozen (30 micron) section after  IF staining and mounting (dried overnight, mounted in dpx after dehydration)? My guess is once it binds it is bound forever. A small piece  with important and nice staining folded on itself, it could be seen only after high mag. Wondering if the section could be somehow separated from fisher brand superfrost plus slide by light steaming or with excess of Triton or else to remount. would appreciate ideas or practical advice,
thanks!

Dr. Igor O. Nasonkin Ph.D.
Postdoctoral Fellow
Pathology/Neuropathology
Johns Hopkins University
Ross Research Bldg Rm 563
720 Rutland Ave
Baltimore MD 21205
tel: 617-388-4104
Igor.Nasonkin <@t> jhmi.edu