[Histonet] Combining Hema 3 and immuno detection
ahaines <@t> vims.edu
Wed Mar 8 16:43:11 CST 2006
Can anyone recommend the best way to combine immunodetection (either via
fluorescence or enzyme) and a differential stain such as Hema 3 (or another
Wright Giemsa like stain)? I'm trying this with cytospins and have had poor
luck. So far either the cell morphology is bad and the signal is good or
the signal is nonexistent but the cell morphology is good!
Thanks in advance!
Ashley N. Haines, PhD.
Postdoctoral Research Associate
Virginia Institute of Marine Science
Department of Environmental & Aquatic Animal Health
PO Box 1346
Gloucester Point, VA 23062
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