[Histonet] Combining Hema 3 and immuno detection

[Ashley Haines]

Hi All,

 

Can anyone recommend the best way to combine immunodetection (either via
fluorescence or enzyme) and a differential stain such as Hema 3 (or another
Wright Giemsa like stain)?  I'm trying this with cytospins and have had poor
luck.  So far either the cell morphology is bad and the signal is good or
the signal is nonexistent but the cell morphology is good!

 

Thanks in advance!

 

Ashley N. Haines, PhD.

Postdoctoral Research Associate

Virginia Institute of Marine Science

Department of Environmental & Aquatic Animal Health

PO Box 1346 

Gloucester Point, VA 23062

804-684-7243