[Histonet] Vital nerve tissue stain (in vivo)

[John Kiernan]

Yes. Methylene blue. There is a large body of
literature dating from Paul Ehrlich (1880s) to the
1990s and perhaps later. Vital staining with
methylene blue should reveal the nerves in a
surgical setting, but you will need to dig in the
literature for practical details. This is a method
that needs intelligence rather than a "protocol."
For my humble contribution see Histochemistry 40:
51-57 (1974). 

John Kiernan
London, Canada
-----------------------------
"UnJa L. Hayes" wrote:
> 
> Dear Histonet,
> 
> Does anyone know of a stain I could use in vivo that would help visualize
> nerves so as to make them easier to locate?
> 
> I need to cut specific nerves in the lower abdominal area of a small
> rodent. However, I'm having difficulty finding the nerves (or all of the
> branches of the nerves). I've searched the web, but couldn't find any simple
> stains that I could use in vivo .
> 
> Any assistance would be greatly appreciated!!!!
> 
> u
> 
> --
> UnJa L. Hayes, Ph.D.
> Center for Neuroendocrine Studies
> University of Massachusetts
> Department of Psychology
> Tobin Hall
> Amherst, MA 01003
> Off: (413) 545-5955
> Lab: (413) 545-0526
> Fax: (413) 545-0996
> 
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