[Histonet] Ki67 on mouse kidney tissue

[Carl Hobbs]

For me, Labvision's SP6 Ki67 worked very well on unfixed, snap-frozen 
sections of mouse skeletal muscle. Fixing sections in 10% formalin in PBS, 
for 10mins, prior to immunostaining gave good reproducible results;  much 
better than methanol:acetone 1:1.
It also works well on perfuse-fixed snap-frozen, cryostat sections.
It works well on pwax for me, also.
NB: The BD-Pharmingen mouse monoclonal Ki67 is equally good; also,  I can 
dilute it by a factor of x1000, rather than  x100  for the Labvision Ab.
You can see pwax pics of the two Abs here http://www.immunoportal.com/ in 
the image gallery