[Histonet] frozen sections

[Charles, Roger]

Try super frost plus slides. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Helen
Beard
Sent: Tuesday, March 07, 2006 5:50 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] frozen sections


   I  have been given some stored mouse brains (-70C), that were fixed
by
   paraformaldehyde  perfusion, cryosectioned, wrapped in foil and
stored
   for  about  a  year. A couple of attempts at IHC on previously cut
and
   stored frozen  sections  and freshly cut sections have resulted in
the
   sections  floating off at various stages. The blocks were difficult
to
   cut (?tissue dried out) with the sections being picked up on
silanised
   slides.  I  tried  air  drying  for  1 hour & air dry/cold
acetone/air
   drying prior to IHC.

   Should  I  air dry for an extended period, try super frost plus
slides
   or  is  the fact that the tissue may have dried out causing most of
my
   difficulties? Any  suggestions  as  to  how I can prevent the
sections
   floating off during IHC would be greatly appreciated.

   Helen
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