[Histonet] frozen sections
hbeard27 <@t> hotmail.com
Tue Mar 7 04:50:24 CST 2006
I have been given some stored mouse brains (-70C), that were fixed by
paraformaldehyde perfusion, cryosectioned, wrapped in foil and stored
for about a year. A couple of attempts at IHC on previously cut and
stored frozen sections and freshly cut sections have resulted in the
sections floating off at various stages. The blocks were difficult to
cut (?tissue dried out) with the sections being picked up on silanised
slides. I tried air drying for 1 hour & air dry/cold acetone/air
drying prior to IHC.
Should I air dry for an extended period, try super frost plus slides
or is the fact that the tissue may have dried out causing most of my
difficulties? Any suggestions as to how I can prevent the sections
floating off during IHC would be greatly appreciated.
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