[Histonet] Revealing lymph nodes

[Marshall Terry Dr, Consultant Histopathologist]

Bob,
All this time on histonet, I never recall reading the imperative of using Davidson's only as a primary fixative.
So, I was not holding my feet right.

Now let's see, how can we organise separate fixative for breast and colon specimens?
Nah, impossible - forget it.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall <@t> rothgen.nhs.uk

-----Original Message-----
From: rsrichmond <@t> aol.com [mailto:rsrichmond <@t> aol.com]
Sent: 03 March 2006 17:23
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Revealing lymph nodes


Terry Marshall notes having had no success with revealing lymph nodes by post-fixation in Davidson's fixative.
 
Neither have I. The clearing fixative must be used as the initial fixative. 
 
An old method is to pass the formalin-fixed tissue through alcohols into xylene (or cedar oil). I've never used this method, and the one time I've seen it used it didn't work. 
 
Once in a while there are no more than three lymph nodes in the axillary contents from a breast cancer. Your surgeon should live with it.
 
Bob Richmond
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