[Histonet] Alcian Blue deposit

[Luck, Greg D.]

Suggestion,
Might try laying the slides down flat, cover with a slightly moist (HOH)
sheet of filter paper (e.g. #40's Whatman) and saturate filter paper with
your Alcian Blue working solution for your standard staining time.  That's
what we do to solve a similar problem with our Gram stain.  Good luck, Greg

-----Original Message-----
From: SARAH REEVES [mailto:SARAH.REEVES <@t> ekht.nhs.uk] 
Sent: Thursday, March 02, 2006 7:37 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Alcian Blue deposit

Hi

Does anyone have any ideas to reduce alcian blue deposit on sections.  We
filter the solution after making up as well as immediatly before use.  

Thanks
Sarah



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