[Histonet] Dako Autostainer

Tom McNemar TMcNemar <@t> lmhealth.org
Wed Jun 28 11:33:32 CDT 2006

What counterstain do you use?  Hematoxylin will clumb and clog the probe causing uneven staining.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Lester
Sent: Wednesday, June 28, 2006 9:34 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Dako Autostainer

Hello, and thanks to all for their input on the PASCAL pressure cooker.  

Another IHC question.  We use the Dako Autostainer Plus and occassionaly have areas on the slides that don't seem to stain.   We are careful not to let the slides dry out.  Do Autostainers have a problem distributing reagents evenly?


Lester J. Raff, MD
Medical Director
UroPartners, LLC Laboratory
ph:  708-486-0076
fax: 708-486-0080

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