[Histonet] CD68 in FFPE mouse tissue

Wed Jun 28 07:49:22 CDT 2006

I am having problems with IHC staining with macrophage-markers in 
formalin-fixed paraffin-embedded (FFPE) mouse arotic root sections. F4/80 
(Serotec) works well in spleen (after proteinase K digestion) but is 
inconsistent in aortas. I have also tried CD68 clone FA11 (Serotec) with 
proteinase K digestion and HIER (citrate, borate, Tris-HCl)  with no 
success. Any tips?
Regards, Einar.