[Histonet] destaining and DAB

Monfils, Paul PMonfils <@t> Lifespan.org
Tue Jun 27 10:31:12 CDT 2006

Destaining the hematoxylin (with acid alcohol?) won't alter the DAB
staining.  DAB staining is extremely stable, and difficult to remove
chemically even when you want to.

> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> cacartwr <@t> mdanderson.org
> Sent: 	Tuesday, June 27, 2006 5:23 AM
> To: 	histonet <@t> lists.utsouthwestern.edu
> Subject: 	[Histonet] destaining and DAB
> I have some slides that I did IHC on with DAB and then I counterstained 
> with Hematoxylin.  I want to destain the hematoxylin and re-do it.  Will 
> destaining affect the DAB?
> Thanks,
> Carrie
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

More information about the Histonet mailing list