[Histonet] histoclear vs xylene

[Smith, Allen]

Although we carefully save our MSDS's in a neat alphabetical file, what we
actually consult is Lewis and Sax's HAZARDOUS CHEMICALS DESK REFERENCE (kept
in my office) or HAZARDOUS PROPERTIES OF INDUSTRIAL MATERIALS (kept in the
lab).  Lewis and Sax say of xylene: "Moderately toxic by inhalation,
ingestion, and subcutaneous routes... Irritation can start at 200 ppm."
Bruce, however is taking due precautions by using xylene in a fume hood.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Anne Van
Binsbergen
Sent: Thursday, June 22, 2006 6:06 AM
To: Bruce Abaloz; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] histoclear vs xylene

ozziebruce
'not considered as toxic'- this level of ignorance NEVER fails to amaze
me.....perhaps you need to do a little more reading up on xylene and
associated harmful effects - there are many!!!!
annieinarabia

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bruce
Abaloz
Sent: Thursday, June 22, 2006 4:28 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] histoclear vs xylene

>John,
>
>I  agree with Rene that Histoclear doesn't work as well as Xylene. 
>We used to deparrafinize routinely in Xylene...X2 changes, 3 min. 
>each.
>Now we use Histolene/Histoclear, x2 changes of 10 min. each - a 
>third wd. probably be good (?) We now have gone back to 
>deparrafinize in Xylene for ALL Immuno/Histochem. 'stuff' (only), as 
>were having problems with 'end results', until - we went back to 
>deparrafinizing in Xylene!
>  As far as the "known health hazards" - check your MSDS/Xylene; it 
>is not even considered Toxic....simply make sure you do all your 
>work under a fumehood/be sensible...I use it to purge my Tissue 
>Processer as - NOTHING works like Xylene.

Cheers,
Bruce in OZ

>





>In a "nutshell" I can tell you the following:
>   1-xylene: universally used for tissue processing and staining but 
>a product that should be avoided because of known health hazards.
>   2-histoclear: a "xylene substitute" sometimes used as a 
>replacement of xylene. Does not work equally well. It is "allegedly 
>safe" but really with no documentation of long term health effects 
>as xylene has. Maybe with time it will turn out to be as hazardous 
>as xylene.
>   Our profession has some health hazards and we just have to work as 
>safely as possible.
>   Hope this will help you!
>
>
>John Campbell
>Graduate Student
>University of Missouri Rolla
>Lab phone 573-341-4069
>Cell phone 573-694-0596
>
>
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-- 
BRUCE ABALOZ 
PH:61383446282
  HISTOLOGIST 
FAX:61383447909
  DEPT. of ZOOLOGY 
EMAIL: brucea <@t> unimelb.edu.au
  THE UNIVERSITY Of MELBOURNE.  VICTORIA. AUSTRALIA 3010         
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Eleanor Roosevelt
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NO-ONE'S WATCHING
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