[Histonet] histoclear vs xylene

[Favara, Cynthia (NIH/NIAID) [E]]

Here is the web site on xylenes from the EPA

http://www.epa.gov/ttn/atw/hlthef/xylenes.html

I would not necessarily agree that "it is not even considered Toxic" but
I do agree nothing is quite like xylene or toluene for histo.

c


Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: Bruce Abaloz [mailto:brucea <@t> unimelb.edu.au] 
Sent: Wednesday, June 21, 2006 5:28 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] histoclear vs xylene

>John,
>
>I  agree with Rene that Histoclear doesn't work as well as Xylene. 
>We used to deparrafinize routinely in Xylene...X2 changes, 3 min. 
>each.
>Now we use Histolene/Histoclear, x2 changes of 10 min. each - a 
>third wd. probably be good (?) We now have gone back to 
>deparrafinize in Xylene for ALL Immuno/Histochem. 'stuff' (only), as 
>were having problems with 'end results', until - we went back to 
>deparrafinizing in Xylene!
>  As far as the "known health hazards" - check your MSDS/Xylene; it 
>is not even considered Toxic....simply make sure you do all your 
>work under a fumehood/be sensible...I use it to purge my Tissue 
>Processer as - NOTHING works like Xylene.

Cheers,
Bruce in OZ

>





>In a "nutshell" I can tell you the following:
>   1-xylene: universally used for tissue processing and staining but 
>a product that should be avoided because of known health hazards.
>   2-histoclear: a "xylene substitute" sometimes used as a 
>replacement of xylene. Does not work equally well. It is "allegedly 
>safe" but really with no documentation of long term health effects 
>as xylene has. Maybe with time it will turn out to be as hazardous 
>as xylene.
>   Our profession has some health hazards and we just have to work as 
>safely as possible.
>   Hope this will help you!
>
>
>John Campbell
>Graduate Student
>University of Missouri Rolla
>Lab phone 573-341-4069
>Cell phone 573-694-0596
>
>
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-- 
BRUCE ABALOZ 
PH:61383446282
  HISTOLOGIST 
FAX:61383447909
  DEPT. of ZOOLOGY 
EMAIL: brucea <@t> unimelb.edu.au
  THE UNIVERSITY Of MELBOURNE.  VICTORIA. AUSTRALIA 3010         
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