[Histonet] IHC with p27?

Dana Settembre settembr <@t> umdnj.edu
Tue Jun 20 12:05:03 CDT 2006

Hello Eva,
I use Dako's p27 Kip 1 and I use it at a dilution of 1:80 with a tonsil
control using Dako's TRS solution in a steamer for 40 minutes.
It looks good.
Good Luck.
Dana Settembre
University Hospital - UMDNJ
Newark, NJ

>>> Eva Andersson <eca9 <@t> georgetown.edu> 06/20/06 9:31 AM >>>
Good morning,
I want to do some IHC with a Kip1/p27 antibody from BD Transduction 
laboratories (610241) but I can't find any references to a working 
concentration. Has anybody used this antibody for IHC? What conditions

did you use?
Thanks for all your help,
Eva Andersson
Research Assistant Georgetown University

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