[Histonet] histo question

[Geoff McAuliffe]

I would think that a Masson's or Mallory's trichrome would do the trick. 
Since most protocols are for human/mammalian tissue you will have to 
fiddle with times. Also, fixation will have an influence on how well the 
stains 'take' so if the tissue is fixed in formalin an overnight soak in 
Bouin's (or just the picric acid part) may be necessary.

Geoff

Campbell, John Thomas (UMR-Student) wrote:

>I am a grad student at the University of Missouri Rolla and I am working on 3D reconstructions of amphibians. We are trying to slice and stain some specimans that we have so we can do the reconstructions. I need to be able to dye the muscle and bone different colors and if possible stain the cartilage too, but this is not neccisary. Does anyone have any suggestions as to what technique I should use?
>
>Thanks
>John
>
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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