[Histonet] IHC with p27?
kappeler <@t> patho.unibe.ch
Tue Jun 20 10:05:32 CDT 2006
We used to use mo-a-Kip1/p27, clone 57, at approx. 1 ug Ig/ml after HIER in
pressure cooker, citrate. As this was several years ago, we did not test
HIER with high pH buffers. Hope this helps.
Institute of Pathology, University of Bern, Switzerland
----- Original Message -----
From: "Eva Andersson" <eca9 <@t> georgetown.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, June 20, 2006 3:31 PM
Subject: [Histonet] IHC with p27?
> Good morning,
> I want to do some IHC with a Kip1/p27 antibody from BD Transduction
> laboratories (610241) but I can't find any references to a working
> concentration. Has anybody used this antibody for IHC? What conditions did
> you use?
> Thanks for all your help,
> Eva Andersson
> Research Assistant Georgetown University
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
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