[Histonet] HMLH1 ON PARAFFIN SECTIONS

[Andi Kappeler]

Seems like they have changed the Cat.Nr. from 554072 to 551091 (clone 
G168-15, however at lower concentration than before). You should find the 
Data Sheet under 
http://www.bdbiosciences.com/external_files/pm/doc/tds/cell_bio/live/web_enabled/1327GN_551091.pdf
Clone G168-15 works significantly better than clone G168-728 (we had a lot 
of trouble with 728) and is no longer recommended for IHC by BD Pharmingen.
Good luck
Andi


----- Original Message ----- 
From: "Malam Jacqueline" <Jacqueline.Malam <@t> rli.mbht.nhs.uk>
To: "'Andi Kappeler'" <kappeler <@t> patho.unibe.ch>
Sent: Tuesday, June 20, 2006 11:46 AM
Subject: RE: [Histonet] HMLH1 ON PARAFFIN SECTIONS


> Many thanks - tried to find it on B&D but was redirected to Clontech who
> didn't have the one you mentioned. I have since found 3 clones at 
> Invitrogen
> and one at BD Pharmingen, clone G168-728, cat no 554073 - could this be 
> the
> same one you mentioned - it's very close. We use the Ventana Benchmark XT 
> so
> plenty of choice when optimising.
> Cheers
> Jacqui
>
> -----Original Message-----
> From: Andi Kappeler [mailto:kappeler <@t> patho.unibe.ch]
> Sent: 20 June 2006 09:53
> To: Malam Jacqueline; Histonet
> Subject: Re: [Histonet] HMLH1 ON PARAFFIN SECTIONS
>
> Hi Jacqui
>
> we use mo-a-hMLH1, clone G168-15, B&D Biosciences Cat.Nr. 554072. Working
> conc. is 2.5 ug Ig/ml after HIER in pressure cooker / citrate. Hope this
> helps.
>
> Andi Kappeler
> Institute of Pathology, University of Bern, Switzerland
>
> ----- Original Message -----
> From: "Malam Jacqueline" <Jacqueline.Malam <@t> rli.mbht.nhs.uk>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Tuesday, June 20, 2006 10:02 AM
> Subject: [Histonet] HMLH1 ON PARAFFIN SECTIONS
>
>
>> Has anyone had any experience of using this antibody on paraffin 
>> sections?
>
>> I
>> have located a supplier (Serotec) - are there any others out there?
>>
>> Jacqui Malam
>> Lancaster
>> UK
>>
>>
>>
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