[Histonet] modified gomori stain
bwhitaker <@t> brownpathology.com
bwhitaker <@t> brownpathology.com
Sun Jun 18 22:01:25 CDT 2006
Katri,
I think that the muscle they were referring to is the modified Gomori's that the neuropath people use. The normal muscle is green when it is done correctly (and they are always looking for 'ragged red fibers').
I have the protocol somewhere... I'll look for it tomorrow when I'm back in the lab.
Bonnie Whitaker
Brown & Associates Medical Laboratories
Houston, TX
Katri Tuomala <katri <@t> cogeco.ca> wrote :
> If you are referring to Gomori's one step trichrome stain, muscle should =
> be=20
> staining red, not green as collagen. See web page=20
> http://stainsfile.info/StainsFile/theory/tri_gen.htm
>
> Katri
>
> Katri Tuomala
> Hamilton, Ontario, Canada
>
> ----- Original Message -----=20
> From: "manal galal" <galalmkh <@t> yahoo.com>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Saturday, June 17, 2006 4:38 AM
> Subject: [Histonet] modified gomori stain
>
>
> > Hi,
> > I need help with modified gomori stain. I cant get the muscle to
> be=
> =20
> > green. Could anyone help me.
> > Dr.
> Man=
> al=20
> > Galal
> > Consultant Pathology
> >
> Institute=
> of=20
> > Neuromotor Disabilities
> > Cairo,
> Egyp=
> t
> >
> > histonet-request <@t> lists.utsouthwestern.edu
> wrote:
> > Send Histonet mailing list submissions to
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> >
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> > or, via email, send a message with subject or body 'help' to
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> >
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> >
> > When replying, please edit your Subject line so it is more specific
> > than "Re: Contents of Histonet digest..."
> >
> >
> > Today's Topics:
> >
> > 1. RE: Trichrome staining and fibrin
> > (Marshall Terry Dr, Consultant Histopathologist)
> > 2. substrates (Perry, Margaret)
> > 3. toe nails (Conlon, Paula F.)
> > 4. Re: substrates (Rene J Buesa)
> > 5. Re: toe nails (Rene J Buesa)
> > 6. Re: toe nails (Chris Pomajzl)
> > 7. ScyTek's serum free pro-block protein (Maria Mejia)
> > 8. RE: toe nails (Anthony F. Boris)
> > 9. Manual cover slip method - DiscoverSlip? (Geoffrey White)
> > 10. Re: substrates (Susan Q Wells)
> > 11. Sphingomyelin (mtitford <@t> aol.com)
> > 12. High profile blades (Snider, Deanna)
> > 13. Mechanical testing-Dallas-Fort Worth (Ashwin Nair)
> >
> >
> > ----------------------------------------------------------------------
> >
> > Message: 1
> > Date: Wed, 14 Jun 2006 13:39:24 +0100
> > From: "Marshall Terry Dr, Consultant Histopathologist"
> >
> > Subject: RE: [Histonet] Trichrome staining and fibrin
> > To: "Bryan Hewlett" , Timo V?is?nen
> > ,
> > Message-ID:
> >
> > Content-Type: text/plain; charset=3D"iso-8859-1"
> >
> > Well, that's telling me:-)
> >
> > All these years, the success I've had with primary mercury fixation (I
> =
> had=20
> > the original article once), and lack of success with anything other
> was=
> =20
> > entirely due to something else, the techs washing too much in one
> insta=
> nce=20
> > and little enough in another.
> > Who would have thought it.
> > However it may explain my observation that "At times it works and at
> ti=
> mes=20
> > not."
> >
> > Actually, the only time you can be confident that you are looking at=20
> > fibrin, is when it is in strands - easily recognised in H&E.
> > To call anything else fibrin on any tinctorial stain is something akin
> =
> to=20
> > a leap of faith.
> >
> > But - I'll get you back re. your "bees can't fly" thing.
> >
> > Take this/that: :-)
> >
> > The "science has proved that bees can't fly" urban myth
> originated in a=
> =20
> > 1934 book by entomologist Antoine Magnan, who discussed a
> mathematical=20
> > equation by Andre Sainte-Lague, an engineer. The equation proved that
> t=
> he=20
> > maximum lift for an aircraft's wings could not be achieved at
> equivalen=
> t=20
> > speeds of a bee. I.e., an airplane the size of a bee, moving as slowly
> =
> as=20
> > a bee, could not fly. Although this did not mean a bee can't fly
> (which=
> =20
> > after all does not have stationary wings like the posited teensy=20
> > aircraft),
> > nevertheless the idea that Magnan's book said bees oughtn't be able to
> =
> fly=20
> > began to spread.
> >
> >
> > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
> > Consultant Pathologist
> > Rotherham General Hospital
> > South Yorkshire
> > England
> > terry.marshall <@t> rothgen.nhs.uk
> >
> > -----Original Message-----
> > From: Bryan Hewlett [mailto:bhewlett <@t> cogeco.ca]
> > Sent: 13 June 2006 17:14
> > To: Marshall Terry Dr, Consultant Histopathologist; Timo V=E4is=E4nen;
> > histonet <@t> lists.utsouthwestern.edu
> > Subject: Re: [Histonet] Trichrome staining and fibrin
> >
> >
> > Terry,
> >
> > Actually NOT!
> > True, Lendrum's original papers(1962) recommended fixation in=20
> > picro-mercuric
> > alcohol for 3 weeks (a tad unnecessary that!).
> > However, both Prof. Lendrum and Bill Slidders also successfully used
> > formaldehyde fixed material that was treated in Bouin immediately
> prior=
> to
> > staining.
> > The key to all of the Masson Type trichromes on formaldehyde-fixed
> tiss=
> ue,
> > is to use a pretreatment in picric acid.
> > This re-aligns the reactive side chains on the proteins, so that there
> =
> is=20
> > a
> > predominance of basic amino groups and hence maximal binding of anionic
> > dyes.
> > Mercury fixation is simply not necessary! Nor is Zinc substitution.
> (se=
> e
> > Troubleshooting Histology stains: Horobin and Bancroft 1998, page 129)
> > The lack of consistency, in obtaining good red colour, is due
> variabili=
> ty=20
> > in
> > the staining procedure (the post dye water rinses), NOT the fixation.
> > Once this is addressed, the inconsistency goes away.
> > That is true for the original Masson, Picro-Mallory (all variants),
> MSB=
> =20
> > and
> > Masson 44/41.
> > Achieving consistent good red colour with formaldehyde fixed material
> i=
> s
> > easy, if the staining mechanisms are understood and those
> unnecessary=20
> > water
> > rinses removed.
> > Been doing it for over 40 years!
> >
> > Bryan
> > (Engineers can prove that the bumble bee simply cannot fly. However,
> th=
> e
> > bumble bee doesn't know that and flies anyway)
> >
> > ----- Original Message -----=20
> > From: "Marshall Terry Dr, Consultant Histopathologist"
> >
> > To: "Bryan Hewlett" ; "Timo V=E4is=E4nen"
> > ;
> > Sent: Tuesday, June 13, 2006 11:27 AM
> > Subject: RE: [Histonet] Trichrome staining and fibrin
> >
> >
> > Bryan,
> >
> > You know full well that MSB required initial staining in mercury. I
> kno=
> w
> > that zinc is an adequate replacement.
> > Attempting to get a consistent good red colour with formalin fixed=20
> > material
> > is futile, as is post-fixation.
> > At times it works and at times not.
> >
> > IMO, Masson is useless for fibrin.
> >
> > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
> > Consultant Pathologist
> > Rotherham General Hospital
> > South Yorkshire
> > England
> > terry.marshall <@t> rothgen.nhs.uk
> >
> > -----Original Message-----
> > From: Bryan Hewlett [mailto:bhewlett <@t> cogeco.ca]
> > Sent: 13 June 2006 15:52
> > To: Timo V=E4is=E4nen; histonet <@t> lists.utsouthwestern.edu
> > Subject: Re: [Histonet] Trichrome staining and fibrin
> >
> >
> > Timo,
> >
> > Try the Lendrum MSB trichrome variant, it was designed to demonstrate
> > fibrin.
> > I will send the method via a separate e-mail.
> >
> > Bryan
> >
> > ----- Original Message -----=20
> > From: "Timo V=E4is=E4nen"
> > To:
> > Sent: Tuesday, June 13, 2006 10:33 AM
> > Subject: [Histonet] Trichrome staining and fibrin
> >
> >
> >> Hello all,
> >>
> >> I have struggled with getting Masson Trichrome (Goldner) staining to
> w=
> ork
> >> with
> >> kidney biopsies. The problem is that our pathologist does not
> get=20
> >> positive
> >> fibrin staining to glomeruli in diseased kidneys. Those areas of
> the
> >> glomeruli
> >> that should contain fibrin, at least according to the pathologist,
> are
> >> green
> >> (Lichtgrun) and not red, as they should be, whatever I do. I have
> trie=
> d=20
> >> to
> >> enhance the staining with Bouin's fixative (+56C) pretreatment but it
> =
> did
> >> not
> >> change the overall situation. However, the red stain was more intense
> =
> in
> >> skin
> >> samples containing fibrin (formalin fixed). I have also tried
> another
> >> Trichrome
> >> staining with Crocein Scarlet 7B without any luck. In our lab
> kidney
> >> biopsies
> >> are routinely fixed with alcoholic Bouin's. As far as I know it
> should=
> be
> >> compatible with the Masson protocol we use. Any ideas? Any good
> protoc=
> ols
> >> that
> >> I could try?
> >>
> >> Thank's in advance,
> >>
> >> Timo
> >>
> >>
> >> _______________________________________________
> >> Histonet mailing list
> >> Histonet <@t> lists.utsouthwestern.edu
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >>
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > ------------------------------
> >
> > Message: 2
> > Date: Wed, 14 Jun 2006 09:08:36 -0500
> > From: "Perry, Margaret"
> > Subject: [Histonet] substrates
> > To:
> > Message-ID:
> >
> > Content-Type: text/plain; charset=3D"us-ascii"
> >
> > We are trying to do double staining and are looking for two different
> > colors of substrate and we can't use DAB due to melanin. Does anyone
> > know of a green stain that is xylene compatible? Vector has Nova Red
> > and a blue color but we use hematoxylin as a counterstain. It would
> > mean an extra step for us to counterstain in Methyl Green
> >
> > We use a DAKO autostainer.
> >
> >
> >
> > Margaret Perry HT(ASCP)
> >
> > South Dakota State University
> >
> > Animal Research and Diagnostic Lab
> >
> > Brookings SD 57007
> >
> > Margaret.Perry <@t> sdstate.edu
> >
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 3
> > Date: Wed, 14 Jun 2006 10:16:10 -0400
> > From: "Conlon, Paula F."
> >
> > Subject: [Histonet] toe nails
> > To: "Histonet \(E-mail\)"
> > Message-ID:
> >
> > Content-Type: text/plain; charset=3D"iso-8859-1"
> >
> > Hi Histonetters, does anyone have a good procedure for sectioning
> nails=
> =20
> > and keeping them on the slides during h&e staining and pas
> staining?Mos=
> t=20
> > of the time our sections wash off. Thanks for your help.
> >
> >
> > See our web page at http://www.lahey.org for a full directory of Lahey=20
> > sites, staff, services and career opportunities.
> >
> > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS=20
> > ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL
> =
> AND=20
> > EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the
> intende=
> d=20
> > recipient, your use of this message for any purpose is strictly=20
> > prohibited. If you have received this communication in error, please=20
> > delete the message and notify the sender so that we may correct our=20
> > records.
> >
> >
> > ------------------------------
> >
> > Message: 4
> > Date: Wed, 14 Jun 2006 07:23:19 -0700 (PDT)
> > From: Rene J Buesa
> > Subject: Re: [Histonet] substrates
> > To: "Perry, Margaret" ,
> > histonet <@t> lists.utsouthwestern.edu
> > Message-ID: <20060614142319.7044.qmail <@t> web61216.mail.yahoo.com>
> > Content-Type: text/plain; charset=3Diso-8859-1
> >
> > Margaret:
> > Confronted with that situation I would eliminate the melanine
> better.=20
> > Oxidize it with 0.1% potassium permanganate for 2 hours; wash and
> remov=
> e=20
> > the oxidized melanine with 0.3% oxalic acid followed by washing. The=20
> > section should be free of melanine pigment/colour afterwards and you
> ca=
> n=20
> > use DAB (for brown) and DAB + Ni salts for dark blue as double
> substrat=
> es.
> > Hope this will help you!
> > Ren=E9 J.
> >
> > "Perry, Margaret" wrote:
> > We are trying to do double staining and are looking for two different
> > colors of substrate and we can't use DAB due to melanin. Does anyone
> > know of a green stain that is xylene compatible? Vector has Nova Red
> > and a blue color but we use hematoxylin as a counterstain. It would
> > mean an extra step for us to counterstain in Methyl Green
> >
> > We use a DAKO autostainer.
> >
> >
> >
> > Margaret Perry HT(ASCP)
> >
> > South Dakota State University
> >
> > Animal Research and Diagnostic Lab
> >
> > Brookings SD 57007
> >
> > Margaret.Perry <@t> sdstate.edu
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> >
> > __________________________________________________
> > Do You Yahoo!?
> > Tired of spam? Yahoo! Mail has the best spam protection around
> > http://mail.yahoo.com
> >
> > ------------------------------
> >
> > Message: 5
> > Date: Wed, 14 Jun 2006 07:25:13 -0700 (PDT)
> > From: Rene J Buesa
> > Subject: Re: [Histonet] toe nails
> > To: "Conlon, Paula F."
> > , "Histonet
> > \(E-mail\)"
> > Message-ID: <20060614142513.15660.qmail <@t> web61219.mail.yahoo.com>
> > Content-Type: text/plain; charset=3Diso-8859-1
> >
> > Paula:
> > I am forwarding privately a summary on Toenails I prepared recently
> tha=
> t I=20
> > hope will answer your questions.
> > Ren=E9 J.
> >
> > "Conlon, Paula F."
> > wrote:
> > Hi Histonetters, does anyone have a good procedure for sectioning
> nails=
> =20
> > and keeping them on the slides during h&e staining and pas
> staining?Mos=
> t=20
> > of the time our sections wash off. Thanks for your help.
> >
> >
> > See our web page at http://www.lahey.org for a full directory of Lahey=20
> > sites, staff, services and career opportunities.
> >
> > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS=20
> > ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL
> =
> AND=20
> > EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the
> intende=
> d=20
> > recipient, your use of this message for any purpose is strictly=20
> > prohibited. If you have received this communication in error, please=20
> > delete the message and notify the sender so that we may correct our=20
> > records.
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> >
> > __________________________________________________
> > Do You Yahoo!?
> > Tired of spam? Yahoo! Mail has the best spam protection around
> > http://mail.yahoo.com
> >
> > ------------------------------
> >
> > Message: 6
> > Date: Wed, 14 Jun 2006 09:41:48 -0500
> > From: "Chris Pomajzl"
> > Subject: Re: [Histonet] toe nails
> > To: "HISTONET"
> > Message-ID: <002e01c68fc0$b2fb6330$26fca8c0 <@t> CSP>
> > Content-Type: text/plain; charset=3D"iso-8859-1"
> >
> > Good question. We have dealt with this issue for many years, and we
> hav=
> e
> > come up with a solution that has worked for some time.
> >
> > First of all, sections are picked up on "+" coated slides that
> have bee=
> n
> > smeared with egg albumin. We separate eggs from the grocery store and
> k=
> eep=20
> > a
> > stock of the egg white albumin in a bottle. Maybe 100ml at a time,
> keep=
> it
> > refrigerated. When cutting nails, put 2-3 drops of albumin on the
> slide=
> =20
> > and
> > smear to cover all of the glass.
> >
> > We cut 3 sections of the nail specimen. 1 for H&E, 1 for PAS, and 1
> ext=
> ra
> > for back-up. We bake the slides at 55'C overnight, and then at 70'C
> for=
> 20
> > minutes prior to staining the following day.
> >
> > This seems to work very well for us.
> >
> > ----- Original Message -----=20
> > From: "Conlon, Paula F."
> >
> > To: "Histonet (E-mail)"
> > Sent: Wednesday, June 14, 2006 9:16 AM
> > Subject: [Histonet] toe nails
> >
> >
> > Hi Histonetters, does anyone have a good procedure for sectioning
> nails=
> =20
> > and
> > keeping them on the slides during h&e staining and pas staining?Most
> of=
> =20
> > the
> > time our sections wash off. Thanks for your help.
> >
> >
> > See our web page at http://www.lahey.org for a full directory of Lahey
> > sites, staff, services and career opportunities.
> >
> > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS=20
> > ADDRESSED.
> > IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND
> EXEMPT=20
> > FROM
> > DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended
> recipient,=
> =20
> > your
> > use of this message for any purpose is strictly prohibited. If you have
> > received this communication in error, please delete the message and
> not=
> ify
> > the sender so that we may correct our records.
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 7
> > Date: Wed, 14 Jun 2006 08:11:41 -0700
> > From: Maria Mejia
> > Subject: [Histonet] ScyTek's serum free pro-block protein
> > To: histonet <@t> lists.utsouthwestern.edu
> > Message-ID:
> > Content-Type: text/plain; charset=3DUS-ASCII; delsp=3Dyes;
> format=3Dflo=
> wed
> >
> > To anyone who using ScyTek's serum free pro-block protein, please let
> > me know
> > what you think of it. In IHC, do you (only) use it as a diluent for
> > the antibodies or also
> > use it in the washes? Any information you can provide will be greatly
> > appreciated.
> >
> > Yours
> >
> > Maria Bartola Mejia
> > UCSF
> > Depart. of Neurosurgery
> > San Francisco, CA 94103
> >
> >
> >
> > ------------------------------
> >
> > Message: 8
> > Date: Wed, 14 Jun 2006 11:51:21 -0400
> > From: "Anthony F. Boris"
> > Subject: RE: [Histonet] toe nails
> > To: "HISTONET"
> > Message-ID:
> > Content-Type: text/plain; charset=3D"utf-8"
> >
> > We use charged slides from Premiere. keep in 65 degree oven for 30
> minu=
> tes=20
> > and stain. We have not had any fall off since we switched to these
> slid=
> es.=20
> > When cutting you can use a 5% solution of KOh for a "surface
> decal". Th=
> is=20
> > breaks down the keratin a little bit (same stuff as in NAIR). Helps to
> =
> get=20
> > decent sectioning.
> >
> > Tony
> >
> > -----Original Message-----=20
> > From: Chris Pomajzl [mailto:cpomajzl <@t> cpllabs.com]
> > Sent: Wed 6/14/2006 10:41 AM
> > To: HISTONET
> > Cc:
> > Subject: Re: [Histonet] toe nails
> >
> >
> >
> > Good question. We have dealt with this issue for many years, and we
> hav=
> e
> > come up with a solution that has worked for some time.
> >
> > First of all, sections are picked up on "+" coated slides that
> have bee=
> n
> > smeared with egg albumin. We separate eggs from the grocery store and
> k=
> eep=20
> > a
> > stock of the egg white albumin in a bottle. Maybe 100ml at a time,
> keep=
> it
> > refrigerated. When cutting nails, put 2-3 drops of albumin on the
> slide=
> =20
> > and
> > smear to cover all of the glass.
> >
> > We cut 3 sections of the nail specimen. 1 for H&E, 1 for PAS, and 1
> ext=
> ra
> > for back-up. We bake the slides at 55'C overnight, and then at 70'C
> for=
> 20
> > minutes prior to staining the following day.
> >
> > This seems to work very well for us.
> >
> > ----- Original Message -----
> > From: "Conlon, Paula F."
> >
> > To: "Histonet (E-mail)"
> > Sent: Wednesday, June 14, 2006 9:16 AM
> > Subject: [Histonet] toe nails
> >
> >
> > Hi Histonetters, does anyone have a good procedure for sectioning
> nails=
> =20
> > and
> > keeping them on the slides during h&e staining and pas staining?Most
> of=
> =20
> > the
> > time our sections wash off. Thanks for your help.
> >
> >
> > See our web page at http://www.lahey.org for a full directory of Lahey
> > sites, staff, services and career opportunities.
> >
> > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS=20
> > ADDRESSED.
> > IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND
> EXEMPT=20
> > FROM
> > DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended
> recipient,=
> =20
> > your
> > use of this message for any purpose is strictly prohibited. If you have
> > received this communication in error, please delete the message and
> not=
> ify
> > the sender so that we may correct our records.
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 9
> > Date: Wed, 14 Jun 2006 08:52:10 -0700 (PDT)
> > From: Geoffrey White
> > Subject: [Histonet] Manual cover slip method - DiscoverSlip?
> > To: histonet <@t> lists.utsouthwestern.edu
> > Message-ID: <20060614155210.68428.qmail <@t> web36513.mail.mud.yahoo.com>
> > Content-Type: text/plain; charset=3Diso-8859-1
> >
> >
> > Dear all,
> >
> >
> >
> > at the time our research group is looking for new manual method for
> cov=
> er=20
> > our slides during the hybridization. In the past we used standard
> glass=
> =20
> > cover slips but we are not really satisfied with this method.
> >
> > Just I saw at Biocompare a new method with the name DiscoverSlip.
> This=20
> > technology looks very interesting.
> >
> > Have any work with DiscoverSlip or saw this technology working?
> >
> >
> >
> > Thanks
> >
> > Geoffrey
> >
> > __________________________________________________
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> > ------------------------------
> >
> > Message: 10
> > Date: Wed, 14 Jun 2006 12:01:42 -0400
> > From: Susan Q Wells
> > Subject: Re: [Histonet] substrates
> > To: Rene J Buesa
> > Cc: histonet <@t> lists.utsouthwestern.edu,
> "Perry, Margaret"
> >
> > Message-ID: <449032E6.8030300 <@t> bms.com>
> > Content-Type: text/plain; format=3Dflowed; charset=3DISO-8859-1
> >
> > You may lose your epitopes with this procedure, if so 10% H202 in PBS
> > for 30 minutes may lighten the melanin pigment enough
> > to view your chromagen. I recently used Vulcan Fast Red and Bajoran
> > Purple from Biocare Medical with great success where melanin pigment
> wa=
> s
> > present.
> >
> > Sue Wells HT(ASCP), QIHC
> >
> > Rene J Buesa wrote:
> >
> >>Margaret:
> >> Confronted with that situation I would eliminate the melanine
> better.=20
> >> Oxidize it with 0.1% potassium permanganate for 2 hours; wash and
> remo=
> ve=20
> >> the oxidized melanine with 0.3% oxalic acid followed by washing.
> The=20
> >> section should be free of melanine pigment/colour afterwards and you
> c=
> an=20
> >> use DAB (for brown) and DAB + Ni salts for dark blue as double=20
> >> substrates.
> >
> > =3D=3D=3D message truncated =3D=3D=3D
> >
> >
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