[Histonet] A Call for Help from Melbourne/OZ
brucea <@t> unimelb.edu.au
Wed Jun 14 23:30:18 CDT 2006
My name is Scott Sinclair, and I am a PhD student in the Department
of Genetics (@ The University of Melbourne/Australia) and am hoping
to get your advice on the following:
I have been using a fluorescent dye (Zinpyr) to try to image Zinc in
the roots of Arabidopsis seedlings/plant, using a confocal
microscope. I'm also interested in looking at paraffin sections of
roots, therefore wondering if there is a 'fixing' process that will
leave the intracellular localisation of Zinc undisturbed while
examining sections/Will routine fixation in 10% NBF or 70% ETOH be
fine? I know you have to fix sections before looking at protein or
DNA/RNA, and was wondering if it can be done for ions also?
Scott Sinclair (sent through Bruce)
DEPT. of ZOOLOGY
EMAIL: brucea <@t> unimelb.edu.au
THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010
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