[Histonet] A Call for Help from Melbourne/OZ

[Bruce Abaloz]

Dear Histonetters,

My name is Scott Sinclair, and I am a PhD student in the Department 
of Genetics (@ The University of Melbourne/Australia) and am hoping 
to get your advice on the following:
I have been using a fluorescent dye (Zinpyr) to try to image Zinc in 
the roots of Arabidopsis seedlings/plant, using a confocal 
microscope. I'm also interested in looking at paraffin sections of 
roots, therefore wondering if there is a 'fixing' process that will 
leave the intracellular localisation of Zinc undisturbed while 
examining sections/Will routine fixation in 10% NBF or 70% ETOH be 
fine? I know you have to fix sections before looking at protein or 
DNA/RNA, and was wondering if it can be done for ions also?

Regards,

Scott Sinclair (sent through Bruce)
-- 
BRUCE ABALOZ 
PH:61383446282
  HISTOLOGIST 
FAX:61383447909
  DEPT. of ZOOLOGY 
EMAIL: brucea <@t> unimelb.edu.au
  THE UNIVERSITY Of MELBOURNE.  VICTORIA. AUSTRALIA 3010         
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