[Histonet] substrates

[Lori Richey]

We  use Giemsa as a counter stain on  tissue with internal  melanin 
pigment.  The Giemsa turns the melanin a dark blue green, which allows 
you to distinguish the brown DAB staining  from the internal melanin 
pigment.  This might be an idea.

Perry, Margaret wrote:

>We are trying to do double staining and are looking for two different
>colors of substrate and we can't use DAB due to melanin.  Does anyone
>know of a green stain that is xylene compatible?  Vector has Nova Red
>and a blue color but we use hematoxylin as a counterstain.  It would
>mean an extra step for us to counterstain in Methyl Green
>
>We use a DAKO autostainer.
>
> 
>
>Margaret Perry HT(ASCP)
>
>South Dakota State University
>
>Animal Research and Diagnostic Lab
>
>Brookings SD 57007
>
>Margaret.Perry <@t> sdstate.edu
>
> 
>
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