[Histonet] toe nails
cpomajzl <@t> cpllabs.com
Wed Jun 14 09:41:48 CDT 2006
Good question. We have dealt with this issue for many years, and we have
come up with a solution that has worked for some time.
First of all, sections are picked up on "+" coated slides that have been
smeared with egg albumin. We separate eggs from the grocery store and keep a
stock of the egg white albumin in a bottle. Maybe 100ml at a time, keep it
refrigerated. When cutting nails, put 2-3 drops of albumin on the slide and
smear to cover all of the glass.
We cut 3 sections of the nail specimen. 1 for H&E, 1 for PAS, and 1 extra
for back-up. We bake the slides at 55'C overnight, and then at 70'C for 20
minutes prior to staining the following day.
This seems to work very well for us.
----- Original Message -----
From: "Conlon, Paula F." <Paula.F.Conlon <@t> Lahey.org>
To: "Histonet (E-mail)" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, June 14, 2006 9:16 AM
Subject: [Histonet] toe nails
Hi Histonetters, does anyone have a good procedure for sectioning nails and
keeping them on the slides during h&e staining and pas staining?Most of the
time our sections wash off. Thanks for your help.
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