Rene J Buesa
rjbuesa <@t> yahoo.com
Wed Jun 14 09:23:19 CDT 2006
Confronted with that situation I would eliminate the melanine better. Oxidize it with 0.1% potassium permanganate for 2 hours; wash and remove the oxidized melanine with 0.3% oxalic acid followed by washing. The section should be free of melanine pigment/colour afterwards and you can use DAB (for brown) and DAB + Ni salts for dark blue as double substrates.
Hope this will help you!
"Perry, Margaret" <Margaret.Perry <@t> sdstate.edu> wrote:
We are trying to do double staining and are looking for two different
colors of substrate and we can't use DAB due to melanin. Does anyone
know of a green stain that is xylene compatible? Vector has Nova Red
and a blue color but we use hematoxylin as a counterstain. It would
mean an extra step for us to counterstain in Methyl Green
We use a DAKO autostainer.
Margaret Perry HT(ASCP)
South Dakota State University
Animal Research and Diagnostic Lab
Brookings SD 57007
Margaret.Perry <@t> sdstate.edu
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