[Histonet] Help with Lung architecture

[Gayle Callis]

The only way we have been able to do this is using Instrumedics Cryojane 
tape transfer device.  It is available in Europe, or go to their website to 
access information.  This is the only way we could get 5 um thin sections 
of air filled lungs.  The instrument is NOT cheap, but is certainly the 
only one we have found that will do this sectioning.  II know people in 
United Kingdom have been able to purchase the instrument.

OR use vibrating microtome sections, these will NOT be thin sections, more 
in the range of 50 um or more but suitable for CLSM and z stack imaging.

  At 09:07 AM 6/6/2006, you wrote:
>Hi,
>
>I am currently searching for a protocol to maintain Lung structure
>intact while avoiding intra tracheal instillation of liquids since I am
>very interested in cells that lie in the outer-most layers of the lung
>(cells that would normally become detached upon lung lavage) Or if such 
>cells remain attached during paraformaldehyde instillation or OCT 
>instillation that would also be very valuable information (I have no idea).
>
>If anyone has any suggestions, or knows how to fix/cut air inflated
>lungs (for example if the trachea is closed before the opening of the
>thoracic cavity or so) I would be extremely grateful for the advise.
>
>The idea is to use these sections for confocal microscopy.
>
>Thank You All in advance.
>
>--
>Alejandro Ortiz Stern M.D. Ph.D.,
>Postdoctoral Fellow
>Institut National de la Santé et de la Recherche Médicale, E0344
>Université de Nice-Sophia-Antipolis
>Institut de Pharmacologie Moléculaire et Cellulaire
>660 Route des Lucioles - 06560 Valbonne - FRANCE
>TEL: 33 (4) 93 95 77 80 - FAX: 33 (4) 93 95 77 08
>
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)