[Histonet] Help with Lung architecture
gcallis <@t> montana.edu
Tue Jun 13 10:40:21 CDT 2006
The only way we have been able to do this is using Instrumedics Cryojane
tape transfer device. It is available in Europe, or go to their website to
access information. This is the only way we could get 5 um thin sections
of air filled lungs. The instrument is NOT cheap, but is certainly the
only one we have found that will do this sectioning. II know people in
United Kingdom have been able to purchase the instrument.
OR use vibrating microtome sections, these will NOT be thin sections, more
in the range of 50 um or more but suitable for CLSM and z stack imaging.
At 09:07 AM 6/6/2006, you wrote:
>I am currently searching for a protocol to maintain Lung structure
>intact while avoiding intra tracheal instillation of liquids since I am
>very interested in cells that lie in the outer-most layers of the lung
>(cells that would normally become detached upon lung lavage) Or if such
>cells remain attached during paraformaldehyde instillation or OCT
>instillation that would also be very valuable information (I have no idea).
>If anyone has any suggestions, or knows how to fix/cut air inflated
>lungs (for example if the trachea is closed before the opening of the
>thoracic cavity or so) I would be extremely grateful for the advise.
>The idea is to use these sections for confocal microscopy.
>Thank You All in advance.
>Alejandro Ortiz Stern M.D. Ph.D.,
>Institut National de la Santé et de la Recherche Médicale, E0344
>Université de Nice-Sophia-Antipolis
>Institut de Pharmacologie Moléculaire et Cellulaire
>660 Route des Lucioles - 06560 Valbonne - FRANCE
>TEL: 33 (4) 93 95 77 80 - FAX: 33 (4) 93 95 77 08
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-4303 (FAX)
More information about the Histonet