[Histonet] ER - fixation

[Bryan Hewlett]

Hi Annette,

I concur with Richard's recommendation re the Goldstein article.
However, I should point out that the suggested minimum 6-8 hours of fixation 
for ER
will only provide approximately 95% confidence for a positive ER result.
Low level ER expressers may still be missed, depending upon your IHC system 
sensitivity.
In addition, 6-8 hours NBF fixation is way too short for reliable HER2 
staining,
and of course you may have to perform IHC for this marker on the same 
material!!
24 hours fixation in NBF at RT, or 18 hours at 37C should be considered the 
minimum time for reliable IHC on breast tumours.

Bryan R. Hewlett
Consultant Technologist
Quality Management Program-Laboratory Services.
Ontario, Canada.



----- Original Message ----- 
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
To: <afeatherstone <@t> kaleidahealth.org>; <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, June 12, 2006 2:38 PM
Subject: [Histonet] ER - fixation


> Hello Annette:
>
> I could not reply to your original e-mail.
>
> Regarding ER and fixation, see Goldstein NS, et al.: Minimum formalin
> fixation time for consistent ER IHC staining of invasive breast
> carcinoma.  Am J Clin Pathol 2003;120:86-92.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 545-1596
> (860) 545-0174 Fax
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>