[Histonet] viability question

[Geoff McAuliffe]

It depends on the storage conditions. If the tissue is stored in a 
physiological buffer with oxygen bubbled through it and somewhere near 
37 degrees C, probably yes. If not ..........?

Geoff

Fawn Jones wrote:

> Dear histonetters,
>
> I am looking for some information for a friend.  She wants to find out 
> if her tissues (aortas) are live or not.  Does anyone have any 
> experience performing viability stains on tissue samples?  She tried 
> the Molecular Probes Live/Dead kit but found it insufficient due to 
> permeabilty issues. She also suggested MTT, but we're not sure how 
> well that stain works with tissues.  Any help would be greatly 
> appreciated.
>
> Thank you
> Fawn Jones HT(ASCP) 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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